ArticleThe Journal of biological chemistry2009
Identification and characterization of the Na+/H+ antiporter Nhas3 from the thylakoid membrane of Synechocystis sp. PCC 6803.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 70 citations in OpenAlex.
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- Na + -driven pH regulation by Na+/H+ antiporters promotes photosynthetic efficiency in cyanobacteria.Plant physiology · 2024Article
- Dissecting structure and function of the monovalent cation/HJournal of bacteriology · 2024Article
- Arbuscular mycorrhizal fungi and salinity stress mitigation in plants.Frontiers in plant science · 2024Review
- The role of the 5' sensing function of ribonuclease E in cyanobacteria.RNA biology · 2024Article
- Antifungal Activity and Alleviation of Salt Stress by Volatile Organic Compounds of NativePlants (Basel, Switzerland) · 2023Article
- The Global Influence of Sodium on Cyanobacteria in Resuscitation from Nitrogen Starvation.Biology · 2023Article
- Chloroplast pH Homeostasis for the Regulation of Photosynthesis.Frontiers in plant science · 2022Review
- Lack of plastid-encoded Ycf10, a homolog of the nuclear-encoded DLDG1 and the cyanobacterial PxcA, enhances the induction of non-photochemical quenching in tobacco.Plant direct · 2021Article
- A Low Level of NaCl Stimulates Plant Growth by Improving Carbon and Sulfur Assimilation inPlants (Basel, Switzerland) · 2021Article
- The Importance of Non-Diffusional Factors in Determining Photosynthesis of Two Contrasting Quinoa Ecotypes (Plants (Basel, Switzerland) · 2021Article
- Geochemical and metagenomics study of a metal-rich, green-turquoise-coloured stream in the southern Swiss Alps.PloS one · 2021Article
- Diverse Physiological Functions of Cation Proton Antiporters across Bacteria and Plant Cells.International journal of molecular sciences · 2020Review
- Current knowledge and recent advances in understanding metabolism of the model cyanobacterium Synechocystis sp. PCC 6803.Bioscience reports · 2020Review
- Improved Salt Tolerance and Metabolomics Analysis ofFrontiers in bioengineering and biotechnology · 2020Article
- Short-Term Temporal Metabolic Behavior in Halophilic CyanobacteriumMetabolites · 2019Article
- Evidence for potassium transport activity of Arabidopsis KEA1-KEA6.Scientific reports · 2019Article
- Characterization of ABC transporter genes, sll1180, sll1181, and slr1270, involved in acid stress tolerance of Synechocystis sp. PCC 6803.Photosynthesis research · 2019Article
- ThePlant physiology · 2017Article
- Proton Gradients and Proton-Dependent Transport Processes in the Chloroplast.Frontiers in plant science · 2016Review
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Authors and funding
15 authors at 8 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Na+/H+ antiporters influence proton or sodium motive force across the membrane. Synechocystis sp. PCC 6803 has six genes encoding Na+/H+ antiporters, nhaS1-5 and sll0556. In this study, the function of NhaS3 was examined. NhaS3 was essential for growth of Synechocystis, and loss of nhaS3 was not complemented by expression of the Escherichia coli Na+/H+ antiporter NhaA. Membrane fractionation followed by immunoblotting as well as immunogold labeling revealed that NhaS3 was localized in the thylakoid membrane of Synechocystis. NhaS3 was shown to be functional over a pH range from pH 6.5 to 9.0 when expressed in E. coli. A reduction in the copy number of nhaS3 in the Synechocystis genome rendered the cells more sensitive to high Na+ concentrations. NhaS3 had no K+/H+ exchange activity itself but enhanced K+ uptake from the medium when expressed in an E. coli potassium uptake mutant. Expression of nhaS3 increased after shifting from low CO2 to high CO2 conditions. Expression of nhaS3 was also found to be controlled by the circadian rhythm. Gene expression peaked at the beginning of subjective night. This coincided with the time of the lowest rate of CO2 consumption caused by the ceasing of O2-evolving photosynthesis. This is the first report of a Na+/H+ antiporter localized in thylakoid membrane. Our results suggested a role of NhaS3 in the maintenance of ion homeostasis of H+, Na+, and K+ in supporting the conversion of photosynthetic products and in the supply of energy in the dark.
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