ArticleNucleic acids research2009
Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 60 citations in OpenAlex.
- A genetic interaction between DED1 and HAT1 in Saccharomyces cerevisiae reveals a role for Hat1p in cytoplasmic RNA granule accumulation.G3 (Bethesda, Md.) · 2026Article
- SIR telomere silencing depends on nuclear envelope lipids and modulates sensitivity to a lysolipid.The Journal of cell biology · 2023Article
- Set4 regulates stress response genes and coordinates histone deacetylases within yeast subtelomeres.Life science alliance · 2021Article
- Gene repression in S. cerevisiae-looking beyond Sir-dependent gene silencing.Current genetics · 2021Review
- Opposing functions of Fng1 and the Rpd3 HDAC complex in H4 acetylation in Fusarium graminearum.PLoS genetics · 2020Article
- The Rad53Nature communications · 2020Article
- Article
- KEOPS complex promotes homologous recombination via DNA resection.Nucleic acids research · 2019Article
- Yeast epigenetics: the inheritance of histone modification states.Bioscience reports · 2019Review
- Epigenetic aspects of telomeric chromatin in Arabidopsis thaliana.BMB reports · 2019Review
- Rpd3L Contributes to the DNA Damage Sensitivity ofGenetics · 2019Article
- Creating a functional single-chromosome yeast.Nature · 2018Article
- The histone methyltransferases Set5 and Set1 have overlapping functions in gene silencing and telomere maintenance.Epigenetics · 2017Article
- Histone Deacetylases with Antagonistic Roles in Saccharomyces cerevisiae Heterochromatin Formation.Genetics · 2016Article
- PMT1 deficiency enhances basal UPR activity and extends replicative lifespan of Saccharomyces cerevisiae.Age (Dordrecht, Netherlands) · 2015Article
- Sirtuins in epigenetic regulation.Chemical reviews · 2015Review
- A sequence-specific interaction between the Saccharomyces cerevisiae rRNA gene repeats and a locus encoding an RNA polymerase I subunit affects ribosomal DNA stability.Molecular and cellular biology · 2015Article
- Curcumin-Mediated HDAC Inhibition Suppresses the DNA Damage Response and Contributes to Increased DNA Damage Sensitivity.PloS one · 2015Article
- Article
- Rpd3- and spt16-mediated nucleosome assembly and transcriptional regulation on yeast ribosomal DNA genes.Molecular and cellular biology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the eukaryotic genome, transcriptionally silent chromatin tends to propagate along a chromosome and encroach upon adjacent active chromatin. The silencing machinery can be stopped by chromatin boundary elements. We performed a screen in Saccharomyces cerevisiae for proteins that may contribute to the establishment of a chromatin boundary. We found that disruption of histone deacetylase Rpd3p results in defective boundary activity, leading to a Sir-dependent local propagation of transcriptional repression. In rpd3 Delta cells, the amount of Sir2p that was normally found in the nucleolus decreased and the amount of Sir2p found at telomeres and at HM and its adjacent loci increased, leading to an extension of silent chromatin in those areas. In addition, Rpd3p interacted directly with chromatin at boundary regions to deacetylate histone H4 at lysine 5 and at lysine 12. Either the mutation of histone H4 at lysine 5 or a decrease in the histone acetyltransferase (HAT) activity of Esa1p abrogated the silencing phenotype associated with rpd3 mutation, suggesting a novel role for the H4 amino terminus in Rpd3p-mediated heterochromatin boundary regulation. Together, these data provide insight into the molecular mechanisms for the anti-silencing functions of Rpd3p during the formation of heterochromatin boundaries.
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