ArticleAnalytical chemistry2009
Combined pulsed-Q dissociation and electron transfer dissociation for identification and quantification of iTRAQ-labeled phosphopeptides.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 32 citations in OpenAlex.
- Quantitative cardiac phosphoproteomics profiling during ischemia-reperfusion in an immature swine model.American journal of physiology. Heart and circulatory physiology · 2017Article
- Improving data quality and preserving HCD-generated reporter ions with EThcD for isobaric tag-based quantitative proteomics and proteome-wide PTM studies.Analytica chimica acta · 2017Article
- Quantitative phosphoproteomics identifies filaggrin and other targets of ionizing radiation in a human skin model.Experimental dermatology · 2012Article
- Discovery- and target-based protein quantification using iTRAQ and pulsed Q collision induced dissociation (PQD).Journal of proteomics · 2012Article
- Sulfonium ion derivatization, isobaric stable isotope labeling and data dependent CID- and ETD-MS/MS for enhanced phosphopeptide quantitation, identification and phosphorylation site characterization.Journal of the American Society for Mass Spectrometry · 2012Article
- Identification of proteins and phosphoproteins using pulsed Q collision induced dissociation (PQD).Journal of the American Society for Mass Spectrometry · 2011Article
- Unraveling pancreatic islet biology by quantitative proteomics.Expert review of proteomics · 2011Review
- Quantitative nuclear proteomics identifies mTOR regulation of DNA damage response.Molecular & cellular proteomics : MCP · 2010Article
- Phosphoproteomics for the masses.ACS chemical biology · 2010Review
Corrections and comments
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Authors and funding
18 authors at 1 institution in 1 country.
Funding
Abstract
Here, we report a new approach that integrates pulsed Q dissociation (PQD) and electron transfer dissociation (ETD) techniques for confident and quantitative identification of iTRAQ-labeled phosphopeptides. The use of isobaric tags for relative and absolute quantification enables a high-throughput quantification of peptides via reporter ion signals in the low m/z range of tandem mass spectra. PQD, a form of ion trap collision activated dissociation, allows for detection of low mass-to-charge fragment ions, and electron transfer dissociation is especially useful for sequencing peptides that contain post-translational modifications. Analysis of the phosphoproteome of human fibroblast cells using a sensitive linear ion trap mass spectrometer demonstrated that this hybrid approach improves both identification and quantification of phosphopeptides. ETD improved phosphopeptide identification, while PQD provides improved quantification of iTRAQ-labeled phosphopeptides.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.