ArticleBiology of reproduction2009
Spermatogonial stem cells derived from infertile Wv/Wv mice self-renew in vitro and generate progeny following transplantation.
Article in Biology of reproduction, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Spermatogonial stem cell technologies: applications from human medicine to wildlife conservation†.Biology of reproduction · 2024Review
- Decoding the spermatogonial stem cell niche under physiological and recovery conditions in adult mice and humans.Science advances · 2023Article
- Primary Cultures of Spermatogonia and Testis Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- In Vitro Culturing of Adult Stem Cells: The Importance of Serum and Atmospheric Oxygen.Advances in experimental medicine and biology · 2022Review
- Xenotransplantation of Human Spermatogonia Into Various Mouse Recipient Models.Frontiers in cell and developmental biology · 2022Article
- Low oxygen tension potentiates proliferation and stemness but not multilineage differentiation of caprine male germline stem cells.Molecular biology reports · 2021Article
- An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia.Genes & development · 2021Article
- Spermatogonial Stem Cell Culture in Oncofertility.The Urologic clinics of North America · 2020Review
- Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein.Stem cell reports · 2020Article
- Article
- Does co-transplantation of mesenchymal and spermatogonial stem cells improve reproductive efficiency and safety in mice?Stem cell research & therapy · 2019Article
- Spermatogonial stem cells.Biology of reproduction · 2018Review
- Conserved and non-conserved characteristics of porcine glial cell line-derived neurotrophic factor expressed in the testis.Scientific reports · 2018Article
- Regulation of blood-testis barrier assembly in vivo by germ cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Long-term health in recipients of transplanted in vitro propagated spermatogonial stem cells.Human reproduction (Oxford, England) · 2018Article
- Spermatogonial stem cell autotransplantation and germline genomic editing: a future cure for spermatogenic failure and prevention of transmission of genomic diseases.Human reproduction update · 2016Review
- A variant of Runx2 that differs from the bone isoform in its splicing is expressed in spermatogenic cells.PeerJ · 2016Article
- c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation.BMC developmental biology · 2013Article
- Restoring fertility in sterile childhood cancer survivors by autotransplanting spermatogonial stem cells: are we there yet?BioMed research international · 2013Review
- White spotting variant mouse as an experimental model for ovarian aging and menopausal biology.Menopause (New York, N.Y.) · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Loss-of-function mutation of the Kit gene causes a severe defect in spermatogenesis that results in infertility due to the inability of its cognate ligand, KIT ligand (KITL), to stimulate spermatogonial proliferation and differentiation. Although self-renewal of mouse spermatogonial stem cells (SSCs) depends on glial cell line-derived neurotrophic factor (GDNF), there is no unequivocal evidence that SSCs with a KIT deficiency can self-renew in vivo or in vitro. In the testis of W(v)/W(v) mice, in which the KIT tyrosine kinase activity is impaired, spermatogonia with SSC phenotype were identified. When W(v)/W(v) spermatogonia were cultured in an SSC culture system supplemented with GDNF in a 10% O(2) atmosphere, they formed clumps and proliferated continuously. An atmosphere of 10% O(2) was better than 21% O(2) to support SSC self-renewal. When W(v)/W(v) clump-forming germ cells were transplanted into testes of infertile wild-type busulfan-treated mice, they colonized the seminiferous tubules but did not differentiate. However, when transplanted into the testes of infertile W/W(v) pups, they restored spermatogenesis and produced spermatozoa, and progeny were generated using microinsemination. These results clearly show that SSCs exist in W(v)/W(v) testes and that they proliferate in vitro similar to wild-type SSCs, indicating that a functional KIT protein is not required for SSC self-renewal. Furthermore, the results indicate that a defect of KIT/KITL signaling of W(v)/W(v) SSCs does not prevent spermatogonial differentiation and spermatogenesis in some recipient strains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.