ArticleJournal of clinical microbiology2009
New real-time PCR-based method for Kingella kingae DNA detection: application to samples collected from 89 children with acute arthritis.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 196 citations in OpenAlex.
- Acute monoarthritis in young children: comparing the characteristics of patients with juvenile idiopathic arthritis versus septic and undifferentiated arthritis.Scientific reports · 2021Trial
- How Should Bacteriological Sampling Be Stratified in Paediatric Septic Arthritis? A Narrative Review with a Proposed Risk-Stratified Framework.Antibiotics (Basel, Switzerland) · 2026Review
- An Evaluation of Novel Inflammatory Biomarkers in Children withPathogens (Basel, Switzerland) · 2026Article
- Osteoarticular Infections in Immunocompetent Children Due to Atypical, Fastidious or Unusual Bacterial Pathogens: A Review.Pathogens (Basel, Switzerland) · 2026Review
- A Swiss Retrospective Case Series of Pediatric Primary Subacute Hematogenous Osteomyelitis.Microorganisms · 2026Article
- A retrospective study of 96 cases comparing x-ray radiography and MRI for diagnosing paediatric subacute osteomyelitis.Frontiers in radiology · 2026Article
- Reflections on so-called culture-negative osteoarticular infections in pediatric populations.Frontiers in pediatrics · 2026Review
- Evaluation of a commercial multiplex pathogen panel for the diagnosis of pediatricJournal of clinical microbiology · 2025Article
- An in-houseJournal of clinical microbiology · 2025Article
- Inefficiency of Kocher and Caird's Criteria in Septic Arthritis of the Hip Due toMicroorganisms · 2025Article
- Performance evaluation of a commercial multiplex pathogen panel for the diagnosis of pediatric joint infections.Journal of clinical microbiology · 2025Article
- An Evaluation of a Syndromic Molecular Panel in Optimising the Microbiological Diagnosis and Antimicrobial Therapy of Suspected Osteoarticular Infections in Paediatric Patients.Diagnostics (Basel, Switzerland) · 2025Article
- Multicenter Retrospective Analysis of 702 Pediatric Cases of Bone and Joint Infections: Definition of Clinical and Biological Features to DiscriminatePathogens (Basel, Switzerland) · 2025Article
- Pathogens in Pediatric Septic Arthritis: A Multi-Center Study in Turkiye (PEDSART Study).Children (Basel, Switzerland) · 2024Article
- Bacteriological diagnosis of osteoarticular infections caused byFrontiers in pediatrics · 2024Review
- Biological Predictors of Osteoarticular Infection Due toMicroorganisms · 2023Article
- Synovial calprotectin: A potentially useful biomarker for the diagnosis ofClinical case reports · 2023Article
- Osteoarticular Infections in Children: Accurately Distinguishing between MSSA and Kingella kingaeMicroorganisms · 2022Article
- Article
- Epidemiology and outcome of septic arthritis in childhood: a 16-year experience and review of literature.Singapore medical journal · 2022Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
14 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inoculation of blood culture vials with joint fluid samples has revealed the important pathogenic role of Kingella kingae in pediatric arthritis. However, recent studies based on broad-range 16S ribosomal DNA PCR and real-time PCR without a probe suggest that conventional methods remain suboptimal. We developed a new real-time PCR method with a probe that is highly specific for K. kingae and applied it to joint fluid samples collected from 89 children with suspected arthritis admitted to our institution during a 2-year period. Real-time PCR was also applied to blood samples obtained before surgery and to joint drainage fluid samples obtained during several days after surgery. Thirty-six (40%) of the 89 cases of suspected septic arthritis had positive culture. Staphylococcus aureus was the main isolate (n = 19/36, 53%), followed by K. kingae (n = 7/36, 19%). Specific real-time PCR identified K. kingae in 24 of the 53 culture-negative cases. Thus, K. kingae was present in 31 (52%) of the 60 documented cases, making it the leading pathogen. Real-time PCR on all 15 blood DNA extracts from patients with K. kingae infection was negative, demonstrating that joint fluid positivity did not result from DNA circulating in blood. Real-time PCR amplification of drainage fluid samples showed that the pathogen could be detected for up to 6 days after antibiotic initiation. K. kingae real-time PCR applied to DNA extracted from joint fluid samples, but not from blood samples, markedly improved the etiological diagnosis of septic arthritis in children. Retrospective diagnosis is feasible for up to 6 days after treatment initiation.
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