ArticleJournal of clinical microbiology2009
Evaluation of the BD GeneOhm StaphSR assay for detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from spiked positive blood culture bottles.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Emerging Diabetic Foot Ulcer Microbiome Analysis Using Cutting Edge Technologies.Journal of diabetes science and technology · 2022Article
- Detection of Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2022Article
- Whole Slide Imaging for High-Throughput Sensing Antibiotic Resistance at Single-Bacterium Level and Its Application to Rapid Antibiotic Susceptibility Testing.Molecules (Basel, Switzerland) · 2019Article
- In vitro antimicrobial susceptibility testing methods: agar dilution to 3D tissue-engineered models.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Review
- Advances in Rapid Identification and Susceptibility Testing of Bacteria in the Clinical Microbiology Laboratory: Implications for Patient Care and Antimicrobial Stewardship Programs.Infectious disease reports · 2017Review
- Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology.Journal of clinical microbiology · 2016Article
- Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now S. aureus and PBP2a Tests.Annals of laboratory medicine · 2015Article
- Article
- Universal Probe Library based real-time PCR for rapid detection of bacterial pathogens from positive blood culture bottles.World journal of microbiology & biotechnology · 2014Article
- Genotypic resistance testing creates new treatment challenges: two cases of oxacillin-susceptible methicillin-resistant Staphylococcus aureus.Journal of clinical microbiology · 2012Article
- Rapid determination of methicillin resistance among Staphylococcus aureus clinical isolates by colorimetric methods.Journal of clinical microbiology · 2012Article
- Impact of results of a rapid Staphylococcus aureus diagnostic test on prescribing of antibiotics for patients with clustered gram-positive cocci in blood cultures.Journal of clinical microbiology · 2012Article
- Bench-to-bedside review: Rapid molecular diagnostics for bloodstream infection--a new frontier?Critical care (London, England) · 2012Review
- Are we ready for novel detection methods to treat respiratory pathogens in hospital-acquired pneumonia?Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2011Review
- Genotypic and phenotypic characterization of methicillin-susceptible Staphylococcus aureus isolates misidentified as methicillin-resistant Staphylococcus aureus by the BD GeneOhm MRSA assay.Journal of clinical microbiology · 2011Article
- Detection of methicillin-resistant Staphylococcus aureus using double duplex real-time PCR and dye Syto 9.Folia microbiologica · 2010Article
- Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry.Journal of clinical microbiology · 2010Article
- Current diagnostic tools for methicillin-resistant Staphylococcus aureus infections.Molecular diagnosis & therapy · 2010Review
- Failure of the BD GeneOhm StaphSR assay for direct detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates in positive blood cultures collected in the United States.Journal of clinical microbiology · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To improve the clinical outcome of Staphylococcus aureus septicemia, the early selection of appropriate antibiotic treatment is crucial. Molecular diagnostics represents an attractive approach for the rapid identification of S. aureus and the determination of its methicillin (meticillin) resistance. In direct comparison to other molecular assays (sa442 and mecA real-time PCRs) and standard laboratory procedures, we evaluated the BD GeneOhm StaphSR assay for its use in the detection of S. aureus and methicillin-resistant S. aureus (MRSA) from spiked blood culture bottles (n = 134). In the case of detecting S. aureus (n = 90; for methicillin-susceptible S. aureus, n = 45; for MRSA, n = 45), the BD GeneOhm StaphSR assay had a sensitivity and a specificity of 100% each (95% confidence intervals [CIs], 96.0 to 100% and 82.4 to 100%, respectively). For MRSA (n = 45), the test was 95.6% (95% CI, 84.9 to 99.5%) sensitive and 95.3% (95% CI, 86.9 to 99.0%) specific. Overall, five discrepant results arose with this assay due to the presence of methicillin-susceptible, revertant MRSA strains (3/45) and MRSA strains that were not detected by the BD GeneOhm StaphSR assay (2/45). Compared to other real-time PCR-based molecular approaches and to conventional standard laboratory methods, the BD GeneOhm StaphSR turned out to be an appropriate diagnostic tool for a rapid (approximately 1.5 h), preliminary identification of S. aureus and MRSA from blood cultures.
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