ArticleMolecular biology of the cell2009
XMAP215-EB1 interaction is required for proper spindle assembly and chromosome segregation in Xenopus egg extract.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Spindle pole proteins confine chromosomes to ensure their expulsion during female meiosis.bioRxiv : the preprint server for biology · 2026Article
- Quantitatively Analyzing the Morphological Growth Dynamics of Bipolar and Non-bipolar Spindles Using Xenopus Egg Extract.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP.Journal of cell science · 2023Article
- RNA localization to the mitotic spindle is essential for early development and is regulated by kinesin-1 and dynein.Journal of cell science · 2023Article
- Tau, XMAP215/Msps and Eb1 co-operate interdependently to regulate microtubule polymerisation and bundle formation in axons.PLoS genetics · 2021Article
- Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.eLife · 2021Article
- Nuclear Isoforms of Neurofibromin Are Required for Proper Spindle Organization and Chromosome Segregation.Cells · 2020Article
- Collective effects of XMAP215, EB1, CLASP2, and MCAK lead to robust microtubule treadmilling.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Microtubule Nucleation Properties of Single Human γTuRCs Explained by Their Cryo-EM Structure.Developmental cell · 2020Article
- Mapping the kinetochore MAP functions required for stabilizing microtubule attachments to chromosomes during metaphase.Cytoskeleton (Hoboken, N.J.) · 2019Review
- Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module.Nature cell biology · 2015Article
- Building the Microtubule Cytoskeleton Piece by Piece.The Journal of biological chemistry · 2015Review
- Drosophila melanogaster mini spindles TOG3 utilizes unique structural elements to promote domain stability and maintain a TOG1- and TOG2-like tubulin-binding surface.The Journal of biological chemistry · 2015Article
- The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array.Molecular biology of the cell · 2014Article
- End-binding protein 1 stimulates paclitaxel sensitivity in breast cancer by promoting its actions toward microtubule assembly and stability.Protein & cell · 2014Article
- Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels.Nature cell biology · 2013Article
- Biochemical evidence that human EB1 does not bind preferentially to the microtubule seam.Cytoskeleton (Hoboken, N.J.) · 2013Article
- New insights into the mechanism for chromosome alignment in metaphase.International review of cell and molecular biology · 2013Review
- Multiple domains of human CLASP contribute to microtubule dynamics and organization in vitro and in Xenopus egg extracts.Cytoskeleton (Hoboken, N.J.) · 2012Article
- SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase.The Journal of cell biology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In metaphase Xenopus egg extracts, global microtubule growth is mainly promoted by two unrelated microtubule stabilizers, end-binding protein 1 (EB1) and XMAP215. Here, we explore their role and potential redundancy in the regulation of spindle assembly and function. We find that at physiological expression levels, both proteins are required for proper spindle architecture: Spindles assembled in the absence of EB1 or at decreased XMAP215 levels are short and frequently multipolar. Moreover, the reduced density of microtubules at the equator of DeltaEB1 or DeltaXMAP215 spindles leads to faulty kinetochore-microtubule attachments. These spindles also display diminished poleward flux rates and, upon anaphase induction, they neither segregate chromosomes nor reorganize into interphasic microtubule arrays. However, EB1 and XMAP215 nonredundantly regulate spindle assembly because an excess of XMAP215 can compensate for the absence of EB1, whereas the overexpression of EB1 cannot substitute for reduced XMAP215 levels. Our data indicate that EB1 could positively regulate XMAP215 by promoting its binding to the microtubules. Finally, we show that disruption of the mitosis-specific XMAP215-EB1 interaction produces a phenotype similar to that of either EB1 or XMAP215 depletion. Therefore, the XMAP215-EB1 interaction is required for proper spindle organization and chromosome segregation in Xenopus egg extracts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.