ArticleMolecular biology of the cell2009
Microtubule cytoskeleton remodeling by acentriolar microtubule-organizing centers at the entry and exit from mitosis in Drosophila somatic cells.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- The role of chromosome/kinetochore-driven microtubule generation in Drosophila spindle assembly.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Article
- SOGA1 and SOGA2/MTCL1 are CLASP-interacting proteins required for faithful chromosome segregation in human cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Article
- Centriole-independent mitotic spindle assembly relies on the PCNT-CDK5RAP2 pericentriolar matrix.The Journal of cell biology · 2020Article
- Review
- Article
- Plk4 Regulates Centriole Asymmetry and Spindle Orientation in Neural Stem Cells.Developmental cell · 2019Article
- Centrosome function and assembly in animal cells.Nature reviews. Molecular cell biology · 2015Review
- Dissecting the function and assembly of acentriolar microtubule organizing centers in Drosophila cells in vivo.PLoS genetics · 2015Article
- Coiled-coil proteins facilitated the functional expansion of the centrosome.PLoS computational biology · 2014Article
- Mitotic spindle multipolarity without centrosome amplification.Nature cell biology · 2014Review
- γ-Tubulin Ring Complexes and EB1 play antagonistic roles in microtubule dynamics and spindle positioning.The EMBO journal · 2014Article
- Synergy between multiple microtubule-generating pathways confers robustness to centrosome-driven mitotic spindle formation.Developmental cell · 2014Article
- Loss of centrioles causes chromosomal instability in vertebrate somatic cells.The Journal of cell biology · 2013Article
- Establishment and mitotic characterization of new Drosophila acentriolar cell lines from DSas-4 mutant.Biology open · 2013Article
- Modulation of Golgi-associated microtubule nucleation throughout the cell cycle.Cytoskeleton (Hoboken, N.J.) · 2013Article
- The centrosome and bipolar spindle assembly: does one have anything to do with the other?Cell cycle (Georgetown, Tex.) · 2011Article
- Differential control of Eg5-dependent centrosome separation by Plk1 and Cdk1.The EMBO journal · 2011Article
- Amphiastral mitotic spindle assembly in vertebrate cells lacking centrosomes.Current biology : CB · 2011Article
- Genetic interaction of centrosomin and bazooka in apical domain regulation in Drosophila photoreceptor.PloS one · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoskeleton microtubules undergo a reversible metamorphosis as cells enter and exit mitosis to build a transient mitotic spindle required for chromosome segregation. Centrosomes play a dominant but dispensable role in microtubule (MT) organization throughout the animal cell cycle, supporting the existence of concurrent mechanisms that remain unclear. Here we investigated MT organization at the entry and exit from mitosis, after perturbation of centriole function in Drosophila S2 cells. We found that several MTs originate from acentriolar microtubule-organizing centers (aMTOCs) that contain gamma-tubulin and require Centrosomin (Cnn) for normal architecture and function. During spindle assembly, aMTOCs associated with peripheral MTs are recruited to acentriolar spindle poles by an Ncd/dynein-dependent clustering mechanism to form rudimentary aster-like structures. At anaphase onset, down-regulation of CDK1 triggers massive formation of cytoplasmic MTs de novo, many of which nucleated directly from aMTOCs. CDK1 down-regulation at anaphase coordinates the activity of Msps/XMAP215 and the kinesin-13 KLP10A to favor net MT growth and stability from aMTOCs. Finally, we show that microtubule nucleation from aMTOCs also occurs in cells containing centrosomes. Our data reveal a new form of cell cycle-regulated MTOCs that contribute for MT cytoskeleton remodeling during mitotic spindle assembly/disassembly in animal somatic cells, independently of centrioles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.