ArticleMolecular biology of the cell2009
Functional overlap of microtubule assembly factors in chromatin-promoted spindle assembly.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Modulation of the cytoskeleton for cancer therapy.Frontiers in cell and developmental biology · 2025Article
- Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes.The Journal of cell biology · 2023Article
- Molecular insight into how γ-TuRC makes microtubules.Journal of cell science · 2021Review
- Article
- Direct observation of branching MT nucleation in living animal cells.The Journal of cell biology · 2019Article
- The XMAP215 Ortholog Alp14 Promotes Microtubule Nucleation in Fission Yeast.Current biology : CB · 2018Article
- XMAP215 is a microtubule nucleation factor that functions synergistically with the γ-tubulin ring complex.Nature cell biology · 2018Article
- Metaphase Spindle Assembly.Biology · 2017Review
- Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module.Nature cell biology · 2015Article
- Microtubule nucleation at the centrosome and beyond.Nature cell biology · 2015Review
- RNA stimulates Aurora B kinase activity during mitosis.PloS one · 2014Article
- Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.Molecular & cellular proteomics : MCP · 2013Article
- XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules.Nature cell biology · 2013Article
- Article
- Review
- Microtubule assembly in meiotic extract requires glycogen.Molecular biology of the cell · 2011Article
- Augmin promotes meiotic spindle formation and bipolarity in Xenopus egg extracts.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Novel binding of the mitotic regulator TPX2 (target protein for Xenopus kinesin-like protein 2) to importin-alpha.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Distinct pathways from centrosomes and chromatin are thought to contribute in parallel to microtubule nucleation and stabilization during animal cell mitotic spindle assembly, but their full mechanisms are not known. We investigated the function of three proposed nucleation/stabilization factors, TPX2, gamma-tubulin and XMAP215, in chromatin-promoted assembly of anastral spindles in Xenopus laevis egg extract. In addition to conventional depletion-add back experiments, we tested whether factors could substitute for each other, indicative of functional redundancy. All three factors were required for microtubule polymerization and bipolar spindle assembly around chromatin beads. Depletion of TPX2 was partially rescued by the addition of excess XMAP215 or EB1, or inhibiting MCAK (a Kinesin-13). Depletion of either gamma-tubulin or XMAP215 was partially rescued by adding back XMAP215, but not by adding any of the other factors. These data reveal functional redundancy between specific assembly factors in the chromatin pathway, suggesting individual proteins or pathways commonly viewed to be essential may not have entirely unique functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.