ArticlePlant & cell physiology2009
Highly sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry: an application for hormone profiling in Oryza sativa.
Article in Plant & cell physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 192 papers, 1 of them a synthesis that pooled it.
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Who cites it
192 citing papers in PubMed, 1 synthesis or guideline pooled it, 503 citations in OpenAlex.
- Role of cytokinins in seed development in pulses and oilseed crops: Current status and future perspective.Frontiers in genetics · 2022Pooled it
- Comparative transcriptomics reveals hormone signaling and MADS-box genes in divergent development of inflorescences and tendrils in grapevine lateral shoots.Plant cell reports · 2026Article
- Rice EMF3 Alleles Adjust Flower Opening Time to Enhance the Seed Setting Rate Under High Temperature Stress.Plant biotechnology journal · 2026Article
- Cytokinin receptor AHK3 influences leaf size by modulating trans-zeatin-type cytokinin levels in xylem.Plant & cell physiology · 2026Article
- Article
- Hormone-centric multi-omics atlas of flower and early fruit development in tomato.Plant communications · 2026Article
- Bioenergy sorghum stems respond to mechanical stimulation with tissue-specific modifications in hormone homeostasis and anatomical traits.Frontiers in plant science · 2026Article
- Local peptide signalling induces stomatal closure under drought stress.Nature communications · 2025Article
- Large-scale single-cell profiling of stem cells identifies redundant regulators of shoot development and yield trait variation.Developmental cell · 2025Article
- Spatiotemporal localization of jasmonate in the regulation of fruit set in tomato.Journal of experimental botany · 2025Article
- Fluctuations in nitrate availability impact cytokinin biosynthesis through histone modifications of IPT3 in Arabidopsis roots for growth acclimation.Plant communications · 2025Article
- The Ionome-Hormone-Flavonoid Network Shapes Genotype-Dependent Yield Adaptation in Sugarcane.Plants (Basel, Switzerland) · 2025Article
- Innovative metabolic reprogramming in rice: unlocking drought resilience through microbial consortia interaction and sustainable agriculture.3 Biotech · 2025Review
- Exploring the Cytokinin Profile ofMetabolites · 2025Article
- A cytokinin response maximum induces and activates bifacial stem cells for radial growth.Nature plants · 2025Article
- Overexpression of housekeeping geneHorticulture research · 2025Article
- Research on the Hormonomics of ThreeAntioxidants (Basel, Switzerland) · 2025Article
- Spatio-Temporal Regulation of Gibberellin Biosynthesis Contributes to Optimal Rhizome Bud Development.Rice (New York, N.Y.) · 2025Article
- Induction of α-amylase and endosperm-imposed seed dormancy: two pioneering papers in gibberellin research.Planta · 2025Review
- A 294 kb deletion causes reduced leaflet size and biomass in pigeonpea.Plant cell reports · 2025Article
132 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a highly sensitive and high-throughput method for the simultaneous analysis of 43 molecular species of cytokinins, auxins, ABA and gibberellins. This method consists of an automatic liquid handling system for solid phase extraction and ultra-performance liquid chromatography (UPLC) coupled with a tandem quadrupole mass spectrometer (qMS/MS) equipped with an electrospray interface (ESI; UPLC-ESI-qMS/MS). In order to improve the detection limit of negatively charged compounds, such as gibberellins, we chemically derivatized fractions containing auxin, ABA and gibberellins with bromocholine that has a quaternary ammonium functional group. This modification, that we call 'MS-probe', makes these hormone derivatives have a positive ion charge and permits all compounds to be measured in the positive ion mode with UPLC-ESI-qMS/MS in a single run. Consequently, quantification limits of gibberellins increased up to 50-fold. Our current method needs <100 mg (FW) of plant tissues to determine phytohormone profiles and enables us to analyze >180 plant samples simultaneously. Application of this method to plant hormone profiling enabled us to draw organ distribution maps of hormone species in rice and also to identify interactions among the four major hormones in the rice gibberellin signaling mutants, gid1-3, gid2-1 and slr1. Combining the results of hormone profiling data with transcriptome data in the gibberellin signaling mutants allows us to analyze relationships between changes in gene expression and hormone metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.