ArticleProceedings of the National Academy of Sciences of the United States of America2009
Single molecule mechanics of the kinesin neck.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Single-molecule experiments reveal the elbow as an essential folding guide in SMC coiled-coil arms.Biophysical journal · 2022Article
- The NEOtrap - en route with a new single-molecule technique.iScience · 2021Review
- Review
- Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes.Frontiers in molecular biosciences · 2020Review
- Molecular mechanics of coiled coils loaded in the shear geometry.Chemical science · 2018Article
- Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.The Journal of biological chemistry · 2016Article
- Fast or Slow, Either Head Can Start the Processive Run of Kinesin-2 KIF3AC.The Journal of biological chemistry · 2016Article
- Folding and assembly of the large molecular machine Hsp90 studied in single-molecule experiments.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Effects of Obstacles on the Dynamics of Kinesins, Including Velocity and Run Length, Predicted by a Model of Two Dimensional Motion.PloS one · 2016Article
- Kinesin-2 KIF3AC and KIF3AB Can Drive Long-Range Transport along Microtubules.Biophysical journal · 2015Article
- Hierarchical cascades of instability govern the mechanics of coiled coils: helix unfolding precedes coil unzipping.Biophysical journal · 2014Article
- Mutational analysis of dimeric linkers in peri- and cytoplasmic domains of histidine kinase DctB reveals their functional roles in signal transduction.Open biology · 2014Article
- Article
- Biochemical and molecular dynamic simulation analysis of a weak coiled coil association between kinesin-II stalks.PloS one · 2012Article
- Article
- Highly anisotropic stability and folding kinetics of a single coiled coil protein under mechanical tension.Journal of the American Chemical Society · 2011Article
- Moving into the cell: single-molecule studies of molecular motors in complex environments.Nature reviews. Molecular cell biology · 2011Review
- Sampling protein form and function with the atomic force microscope.Molecular & cellular proteomics : MCP · 2010Review
- Fast and forceful refolding of stretched alpha-helical solenoid proteins.Biophysical journal · 2010Article
- Full distance-resolved folding energy landscape of one single protein molecule.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Structural integrity as well as mechanical stability of the parts of a molecular motor are crucial for its function. In this study, we used high-resolution force spectroscopy by atomic force microscopy to investigate the force-dependent opening kinetics of the neck coiled coil of Kinesin-1 from Drosophila melanogaster. We find that even though the overall thermodynamic stability of the neck is low, the average opening force of the coiled coil is >11 pN when stretched with pulling velocities >150 nm/s. These high unzipping forces ensure structural integrity during motor motion. The high mechanical stability is achieved through a very narrow N-terminal unfolding barrier if compared with a conventional leucine zipper. The experimentally mapped mechanical unzipping profile allows direct assignment of distinct mechanical stabilities to the different coiled-coil subunits. The coiled-coil sequence seems to be tuned in an optimal way to ensure both mechanical stability as well as motor regulation through charged residues.
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Registered trials
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