ArticleBMC complementary and alternative medicine2009
Low infra red laser light irradiation on cultured neural cells: effects on mitochondria and cell viability after oxidative stress.
Article in BMC complementary and alternative medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Pulsed Near Infrared Transcranial and Intranasal Photobiomodulation Significantly Modulates Neural Oscillations: a pilot exploratory study.Scientific reports · 2019Trial
- Longevity concept by regenerative medicine methods synergy: exosome therapy, functional medicine, and advanced multi-wavelengths laser therapy.European journal of translational myology · 2025Article
- The Effect of Photobiomodulation on Sperm Parameters and Apoptosis in an Experimental Testicular Torsion Model.Journal of lasers in medical sciences · 2025Article
- PC12 differentiation to neuron cells activated by a low-level laser at 660 nm on UV pre-treated CR-39 scaffolds with parallel microchannels.Biomedical optics express · 2024Article
- Protocol Report on the Transcranial Photobiomodulation for Alzheimer's Disease (TRAP-AD) Study.Healthcare (Basel, Switzerland) · 2023Article
- Photobiomodulation at Defined Wavelengths Regulates Mitochondrial Membrane Potential and Redox Balance in Skin Fibroblasts.Oxidative medicine and cellular longevity · 2023Article
- Photobiomodulation and Sida tuberculata combination declines the inflammation's markers in knee-induced osteoarthritis.Lasers in medical science · 2022Article
- Photobiomodulation reduces hippocampal apoptotic cell death and produces a Raman spectroscopic "signature".PloS one · 2022Article
- Effect of low power lasers on prokaryotic and eukaryotic cells under different stress condition: a review of the literature.Lasers in medical science · 2021Review
- The effects of 808-nm near-infrared laser light irradiation on actin cytoskeleton reorganization in bone marrow mesenchymal stem cells.Cell and tissue research · 2021Article
- Oxidative stress is involved in LLLT mechanism of action on skin healing in rats.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2021Article
- Changes in Brain Function and Structure After Self-Administered Home Photobiomodulation Treatment in a Concussion Case.Frontiers in neurology · 2020Article
- Ultra-Low-Level Laser Therapy and Acupuncture Libralux: What Is so Special?Medicines (Basel, Switzerland) · 2019Article
- Brain Photobiomodulation Therapy: a Narrative Review.Molecular neurobiology · 2018Review
- Transcranial LED therapy on amyloid-β toxin 25-35 in the hippocampal region of rats.Lasers in medical science · 2017Article
- COLasers in medical science · 2017Article
- Far-infrared radiation protects viability in a cell model of Spinocerebellar Ataxia by preventing polyQ protein accumulation and improving mitochondrial function.Scientific reports · 2016Article
- Review of transcranial photobiomodulation for major depressive disorder: targeting brain metabolism, inflammation, oxidative stress, and neurogenesis.Neurophotonics · 2016Review
- In vitro exposure to very low-level laser modifies expression level of extracellular matrix protein RNAs and mitochondria dynamics in mouse embryonic fibroblasts.BMC complementary and alternative medicine · 2015Article
- Protective effect of 940 nm laser on gamma-irradiated mice.Photomedicine and laser surgery · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConsiderable interest has been aroused in recent years by the well-known notion that biological systems are sensitive to visible light. With clinical applications of visible radiation in the far-red to near-infrared region of the spectrum in mind, we explored the effect of coherent red light irradiation with extremely low energy transfer on a neural cell line derived from rat pheochromocytoma. We focused on the effect of pulsed light laser irradiation vis-à-vis two distinct biological effects: neurite elongation under NGF stimulus on laminin-collagen substrate and cell viability during oxidative stress.
methodsWe used a 670 nm laser, with extremely low peak power output (3 mW/cm2) and at an extremely low dose (0.45 mJ/cm2). Neurite elongation was measured over three days in culture. The effect of coherent red light irradiation on cell reaction to oxidative stress was evaluated through live-recording of mitochondria membrane potential (MMP) using JC1 vital dye and laser-confocal microscopy, in the absence (photo bleaching) and in the presence (oxidative stress) of H2O2, and by means of the MTT cell viability assay.
resultsWe found that laser irradiation stimulates NGF-induced neurite elongation on a laminin-collagen coated substrate and protects PC12 cells against oxidative stress.
conclusionThese data suggest that red light radiation protects the viability of cell culture in case of oxidative stress, as indicated by MMP measurement and MTT assay. It also stimulates neurite outgrowth, and this effect could also have positive implications for axonal protection.
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