ArticleNano letters2008
Peeling single-stranded DNA from graphite surface to determine oligonucleotide binding energy by force spectroscopy.
Article in Nano letters, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 187 citations in OpenAlex.
- Interplay of graphene-DNA interactions: Unveiling sensing potential of graphene materials.Applied physics reviews · 2024Article
- Rapid and High-Throughput SARS-CoV-2 RNA Detection without RNA Extraction and Amplification by Using a Microfluidic Biochip.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Single DNA Translocation and Electrical Characterization Based on Atomic Force Microscopy and Nanoelectrodes.IEEE open journal of nanotechnology · 2022Article
- Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application.Biosensors · 2021Article
- Sugar-Pucker Force-Induced Transition in Single-Stranded DNA.International journal of molecular sciences · 2021Article
- Graphene-Based FRET Aptasensors.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Review
- Spectral-Phase Interferometry Detection of Ochratoxin A via Aptamer-Functionalized Graphene Coated Glass.Nanomaterials (Basel, Switzerland) · 2021Article
- Mechanistic insight into biopolymer induced iron oxide mineralization through quantification of molecular bonding.Nanoscale advances · 2020Article
- Graphene Quantum Dot Oxidation Governs Noncovalent Biopolymer Adsorption.Scientific reports · 2020Article
- Step-defect guided delivery of DNA to a graphene nanopore.Nature nanotechnology · 2019Article
- Insight into the DNA adsorption on nitrogen-doped positive carbon dots.RSC advances · 2019Article
- Conformations and cryo-force spectroscopy of spray-deposited single-strand DNA on gold.Nature communications · 2019Article
- Direct Evidence for the Polymeric Nature of Polydopamine.Angewandte Chemie (International ed. in English) · 2019Article
- A graphene aptasensor for biomarker detection in human serum.Electrochimica acta · 2018Article
- Angle-Dependent Atomic Force Microscopy Single-Chain Pulling of Adsorbed Macromolecules from Planar Surfaces Unveils the Signature of an Adsorption-Desorption Transition.Journal of the American Chemical Society · 2018Article
- Size-Dependent Affinity of Glycine and Its Short Oligomers to Pyrite Surface: A Model for Prebiotic Accumulation of Amino Acid Oligomers on a Mineral Surface.International journal of molecular sciences · 2018Article
- Fluorometric determination of lead(II) and mercury(II) based on their interaction with a complex formed between graphene oxide and a DNAzyme.Mikrochimica acta · 2017Article
- Real-Time Monitoring of Insulin Using a Graphene Field-Effect Transistor Aptameric Nanosensor.ACS applied materials & interfaces · 2017Article
- Probe Microscopic Studies of DNA Molecules on Carbon Nanotubes.Nanomaterials (Basel, Switzerland) · 2016Review
- A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor.Sensors (Basel, Switzerland) · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We measured the force required to peel single-stranded DNA molecules from single-crystal graphite using chemical force microscopy. Force traces during retraction of a tip chemically modified with oligonucleotides displayed characteristic plateaus with abrupt force jumps, which we interpreted as a steady state peeling process punctuated by complete detachment of one or more molecules. We were able to differentiate between bases in pyrimidine homopolymers; peeling forces were 85.3 - 4.7 pN for polythymine and 60.8 +/- 5.5 pN for polycytosine, substantially independent of salt concentration and the rate of detachment. We developed a model for peeling a freely jointed chain from the graphite surface and estimated the average binding energy per monomer to be 11.5 +/- 0.6 k(B)T and 8.3 +/- 0.7 k(B)T in the cases of thymine and cytosine nucleotides, respectively. The equilibrium free-energy profile simulated using molecular dynamics had a potential well of 18.9 k(B)T for thymidine, showing that nonelectrostatic interactions dominate the binding. The discrepancy between the experiment and theory indicates that not all bases are adsorbed on the surface or that there is a population of conformations in which they adsorb. Force spectroscopy using oligonucleotides covalently linked to AFM tips provides a flexible and unambiguous means to quantify the strength of interactions between DNA and a number of substrates, potentially including nanomaterials such as carbon nanotubes.
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