ArticlePloS one2009
A novel method for detection of phosphorylation in single cells by surface enhanced Raman scattering (SERS) using composite organic-inorganic nanoparticles (COINs).
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Highly Sensitive, Robust, and Recyclable TiOMolecules (Basel, Switzerland) · 2022Article
- Surface-Enhanced Raman Spectroscopy for Cancer Immunotherapy Applications: Opportunities, Challenges, and Current Progress in Nanomaterial Strategies.Nanomaterials (Basel, Switzerland) · 2020Review
- SERS detection of Biomolecules at Physiological pH via aggregation of Gold Nanorods mediated by Optical Forces and Plasmonic Heating.Scientific reports · 2016Article
- Kinase phosphorylation monitoring with i-motif DNA cross-linked SERS probes.Chemical communications (Cambridge, England) · 2016Article
- A correlative optical microscopy and scanning electron microscopy approach to locating nanoparticles in brain tumors.Micron (Oxford, England : 1993) · 2015Article
- Rapid clinical bacteriology and its future impact.Annals of laboratory medicine · 2013Review
- Raman Labeled Nanoparticles: Characterization of Variability and Improved Method for Unmixing.Journal of Raman spectroscopy : JRS · 2012Article
- Structured penalties for functional linear models-partially empirical eigenvectors for regression.Electronic journal of statistics · 2012Article
- Functionalized arrays of Raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood.Nature communications · 2011Article
- Genomes, proteomes, and the central dogma.Circulation. Cardiovascular genetics · 2011Article
- Sombrero-shaped plasmonic nanoparticles with molecular-level sensitivity and multifunctionality.ACS nano · 2011Article
- Preclinical evaluation of Raman nanoparticle biodistribution for their potential use in clinical endoscopy imaging.Small (Weinheim an der Bergstrasse, Germany) · 2011Article
- Raman chemical imaging of chromate reduction sites in a single bacterium using intracellularly grown gold nanoislands.ACS nano · 2011Article
- Systems biology in immunology: a computational modeling perspective.Annual review of immunology · 2011Review
- Surface-enhanced Raman scattering (SERS) cytometry.Methods in cell biology · 2011Article
- High throughput single nanoparticle spectroscopy.ACS nano · 2009Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
backgroundDetection of single cell epitopes has been a mainstay of immunophenotyping for over three decades, primarily using fluorescence techniques for quantitation. Fluorescence has broad overlapping spectra, limiting multiplexing abilities. METHODOLOGY/PRINCIPAL
findingsTo expand upon current detection systems, we developed a novel method for multi-color immuno-detection in single cells using "Composite Organic-Inorganic Nanoparticles" (COINs) Raman nanoparticles. COINs are Surface-Enhanced Raman Scattering (SERS) nanoparticles, with unique Raman spectra. To measure Raman spectra in single cells, we constructed an automated, compact, low noise and sensitive Raman microscopy device (Integrated Raman BioAnalyzer). Using this technology, we detected proteins expressed on the surface in single cells that distinguish T-cells among human blood cells. Finally, we measured intracellular phosphorylation of Stat1 (Y701) and Stat6 (Y641), with results comparable to flow cytometry. CONCLUSIONS/SIGNIFICANCE: Thus, we have demonstrated the practicality of applying COIN nanoparticles for measuring intracellular phosphorylation, offering new possibilities to expand on the current fluorescent technology used for immunoassays in single cells.
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