ArticleJournal of cell science2009
LC3-mediated fibronectin mRNA translation induces fibrosarcoma growth by increasing connective tissue growth factor.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- LC3B is an RNA-binding protein to trigger rapid mRNA degradation during autophagy.Nature communications · 2022Article
- Discoidin Domain Receptors, DDR1b and DDR2, Promote Tumour Growth within Collagen but DDR1b Suppresses Experimental Lung Metastasis in HT1080 Xenografts.Scientific reports · 2020Article
- Identification of a noncanonical RNA binding domain in the U2 snRNP protein SF3A1.RNA (New York, N.Y.) · 2019Article
- Nuclear LC3 Associates with Slowly Diffusing Complexes that Survey the Nucleolus.Traffic (Copenhagen, Denmark) · 2016Article
- Reduced BMPR2 expression induces GM-CSF translation and macrophage recruitment in humans and mice to exacerbate pulmonary hypertension.The Journal of experimental medicine · 2014Article
- Oncogene-targeting T cells reject large tumors while oncogene inactivation selects escape variants in mouse models of cancer.Cancer cell · 2011Article
- Carbon monoxide activates autophagy via mitochondrial reactive oxygen species formation.American journal of respiratory cell and molecular biology · 2011Article
- Autophagic protein LC3B confers resistance against hypoxia-induced pulmonary hypertension.American journal of respiratory and critical care medicine · 2011Article
- Autophagy in cigarette smoke-induced chronic obstructive pulmonary disease.Expert review of respiratory medicine · 2010Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
Abstract
Previously, we related fibronectin (Fn1) mRNA translation to an interaction between an AU-rich element in the Fn1 3' UTR and light chain 3 (LC3) of microtubule-associated proteins 1A and 1B. Since human fibrosarcoma (HT1080) cells produce little fibronectin and LC3, we used these cells to investigate how LC3-mediated Fn1 mRNA translation might alter tumor growth. Transfection of HT1080 cells with LC3 enhanced fibronectin mRNA translation. Using polysome analysis and RNA-binding assays, we show that elevated levels of translation depend on an interaction between a triple arginine motif in LC3 and the AU-rich element in Fn1 mRNA. Wild-type but not mutant LC3 accelerated HT1080 cell growth in culture and when implanted in SCID mice. Comparison of WT LC3 with vector-transfected HT1080 cells revealed increased fibronectin-dependent proliferation, adhesion and invasion. Microarray analysis of genes differentially expressed in WT and vector-transfected control cells indicated enhanced expression of connective tissue growth factor (CTGF). Using siRNA, we show that enhanced expression of CTGF is fibronectin dependent and that LC3-mediated adhesion, invasion and proliferation are CTGF dependent. Expression profiling of soft tissue tumors revealed increased expression of both LC3 and CTGF in some locally invasive tumor types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.