ArticleProceedings of the National Academy of Sciences of the United States of America2009
Electromechanical coupling in the membranes of Shaker-transfected HEK cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 46 citations in OpenAlex.
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- Mechanical stimulation and electrophysiological monitoring at subcellular resolution reveals differential mechanosensation of neurons within networks.Nature nanotechnology · 2024Article
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- Paradigms of endothelial stiffening in cardiovascular disease and vascular aging.Frontiers in physiology · 2022Review
- Reply to Farrell: Experimental evidence is the ultimate judge for model assumptions.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Plasmonic imaging of subcellular electromechanical deformation in mammalian cells.Journal of biomedical optics · 2019Article
- Hypotonic Challenge of Endothelial Cells Increases Membrane Stiffness with No Effect on Tether Force.Biophysical journal · 2018Article
- Mechanical Transduction and the Dark Energy of Biology.Biophysical journal · 2018Review
- Membrane Stiffening in Osmotic Swelling: Analysis of Membrane Tension and Elastic Modulus.Current topics in membranes · 2018Article
- Response to Alternating Electric Fields of Tubulin Dimers and Microtubule Ensembles in Electrolytic Solutions.Scientific reports · 2017Article
- Study of the union method of microelectrode array and AFM for the recording of electromechanical activities in living cardiomyocytes.European biophysics journal : EBJ · 2017Article
- Adaptation Independent Modulation of Auditory Hair Cell Mechanotransduction Channel Open Probability Implicates a Role for the Lipid Bilayer.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension.Nature communications · 2016Article
- Mechanical transduction by ion channels: A cautionary tale.World journal of neurology · 2015Article
- Mechanical dynamics in live cells and fluorescence-based force/tension sensors.Biochimica et biophysica acta · 2015Review
- Cell volume control in three dimensions: Water movement without solute movement.The Journal of general physiology · 2015Article
- Fluorescence-based force/tension sensors: a novel tool to visualize mechanical forces in structural proteins in live cells.Antioxidants & redox signaling · 2014Review
- Cellular biophysical dynamics and ion channel activities detected by AFM-based nanorobotic manipulator in insulinoma β-cells.Nanomedicine : nanotechnology, biology, and medicine · 2013Article
- Molecular force transduction by ion channels: diversity and unifying principles.Journal of cell science · 2012Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membranes flex with changes in transmembrane potential as a result of changes in interfacial tension, the Lippman effect. We studied the membrane electromotility of Shaker K(+)-transfected HEK-293 cells in real time by using combined patch-clamp atomic force microscopy. In the voltage range where the channels were closed, Shaker expression had little effect on electromotility relative to wild-type cells. Depolarization between -120 and -40 mV resulted in a linear upward cantilever deflection equivalent to an increase in membrane tension. However, when depolarized sufficiently for channel opening, the electromotility saturated and only recovered over 10 s of milliseconds. This remarkable loss of motility was associated with channel opening, not ionic flux or movement of the voltage sensors. The IL mutant of Shaker, in which the voltage dependence of channel opening but not sensor movement is shifted to more positive potentials, caused the loss of electromotility saturation also to shift to more positive potentials. The temporary loss of electromotility associated with channel opening is probably caused by local buckling of the bilayer as the inner half of the channel expands as expected from X-ray structural data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.