ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2009
SIMPLE: a sequential immunoperoxidase labeling and erasing method.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 98 papers.
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Who cites it
98 citing papers in PubMed.
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- Association between PLVAP downregulation and increased lymphocyte infiltration in newly diagnosed glioblastoma treated with bevacizumab.Brain tumor pathology · 2026Article
- Cyclic immunofluorescence platform using photocleavable linkers for direct antibody labeling enables cancer phenotyping.bioRxiv : the preprint server for biology · 2026Article
- Multiple opsin expression in cubozoan ocelli indicates functional redundancy.Scientific reports · 2026Article
- Integrative analysis of T cell subset-specific ICOS expression and tumor HLA class I/II expression in lung adenocarcinoma: implications for their interaction and clinical outcome.Cancer immunology, immunotherapy : CII · 2026Article
- Rhinovirus Infects B and CD4 T Lymphocytes in Hypertrophic Tonsils in Children.Journal of medical virology · 2026Article
- From Stains to Spectra: Multiplexed Immunohistochemistry and Mass Spectrometry Imaging for Spatial Proteomics in Biomedicine.Advances in experimental medicine and biology · 2026Review
- Impact of HTLV-1 infection on clinicopathological characteristics and tumour immune microenvironment in colorectal cancer.Virchows Archiv : an international journal of pathology · 2025Article
- Hippo pathway controls biopterin metabolism to shield adjacent cells from ferroptosis in lung cancer.EMBO reports · 2025Article
- Lymph node macrophage-targeted interferon alpha boosts anticancer immune responses by regulating CD169-positive phenotype of macrophages.Molecular cancer · 2025Article
- Bi-level graph learning unveils prognosis-relevant tumor microenvironment patterns in breast multiplexed digital pathology.Patterns (New York, N.Y.) · 2025Article
- Advances in molecular epidemiology and detection methods of pseudorabies virus.Discover nano · 2025Review
- A machine learning based algorithm accurately stages liver disease by quantification of arteries.Scientific reports · 2025Article
- Chemotherapy Drives Tertiary Lymphoid Structures That Correlate with ICI-Responsive TCF1+CD8+ T Cells in Metastatic Ovarian Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2025Article
- Lymphocyte exhaustion in hepatocellular carcinoma: a dynamic evolution across disease stages.Frontiers in immunology · 2025Article
- Combined Autophagy Inhibition and Dendritic Cell Recruitment Induces Antitumor Immunity and Enhances Immune Checkpoint Blockade Sensitivity in Pancreatic Cancer.Cancer research · 2024Article
- Spatial analysis by current multiplexed imaging technologies for the molecular characterisation of cancer tissues.British journal of cancer · 2024Review
- Tertiary lymphoid structures and B cells determine clinically relevant T cell phenotypes in ovarian cancer.Nature communications · 2024Article
38 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to simultaneously visualize expression of multiple antigens in cells and tissues can provide powerful insights into cellular and organismal biology. However, standard methods are limited to the use of just two or three simultaneous probes and have not been widely adopted for routine use in paraffin-embedded tissue. We have developed a novel approach called sequential immunoperoxidase labeling and erasing (SIMPLE) that enables the simultaneous visualization of at least five markers within a single tissue section. Utilizing the alcohol-soluble peroxidase substrate 3-amino-9-ethylcarbazole, combined with a rapid non-destructive method for antibody-antigen dissociation, we demonstrate the ability to erase the results of a single immunohistochemical stain while preserving tissue antigenicity for repeated rounds of labeling. SIMPLE is greatly facilitated by the use of a whole-slide scanner, which can capture the results of each sequential stain without any information loss.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.