ArticleThe Journal of biological chemistry2009
In situ measurement of airway surface liquid [K+] using a ratioable K+-sensitive fluorescent dye.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Potassium Channels of the Airway Epithelium.Acta physiologica (Oxford, England) · 2026Review
- Redetermined structure of methyl 3-{4,4-di-fluoro-2-[2-(methoxy-car-bon-yl)-ethyl]-1,3,5,7-tetra-methyl-4-bora-3a,4a-di-aza-IUCrData · 2024Article
- Potentiation of BKCa channels by cystic fibrosis transmembrane conductance regulator correctors VX-445 and VX-121.The Journal of clinical investigation · 2024Article
- ATP12A Proton Pump as an Emerging Therapeutic Target in Cystic Fibrosis and Other Respiratory Diseases.Biomolecules · 2023Review
- A simple method to generate human airway epithelial organoids with externally orientated apical membranes.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- Genetic determinants of ammonia-induced acute lung injury in mice.American journal of physiology. Lung cellular and molecular physiology · 2021Article
- Osmotic Adaptation by NaBiomedicines · 2020Review
- Paracellular bicarbonate flux across human cystic fibrosis airway epithelia tempers changes in airway surface liquid pH.The Journal of physiology · 2020Article
- The Effects of the Anti-aging Protein Klotho on Mucociliary Clearance.Frontiers in medicine · 2019Article
- ATP12A promotes mucus dysfunction during Type 2 airway inflammation.Scientific reports · 2018Article
- Esomeprazole Increases Airway Surface Liquid pH in Primary Cystic Fibrosis Epithelial Cells.Frontiers in pharmacology · 2018Article
- Role of Smad3 and p38 Signalling in Cigarette Smoke-induced CFTR and BK dysfunction in Primary Human Bronchial Airway Epithelial Cells.Scientific reports · 2017Article
- Airway Hydration, Apical K(+) Secretion, and the Large-Conductance, Ca(2+)-activated and Voltage-dependent Potassium (BK) Channel.Annals of the American Thoracic Society · 2016Review
- A spatial model of fluid recycling in the airways of the lung.Journal of theoretical biology · 2015Article
- IFN-γ-mediated reduction of large-conductance, Ca2+-activated, voltage-dependent K+ (BK) channel activity in airway epithelial cells leads to mucociliary dysfunction.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Physiology of epithelial chloride and fluid secretion.Cold Spring Harbor perspectives in medicine · 2012Article
- A role for two-pore K⁺ channels in modulating Na⁺ absorption and Cl⁻ secretion in normal human bronchial epithelial cells.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- In vivo determination of mouse olfactory mucus cation concentrations in normal and inflammatory states.PloS one · 2012Article
- A new highly selective fluorescent K+ sensor.Journal of the American Chemical Society · 2011Article
- Triazacryptand-based fluorescent sensors for extracellular and intracellular K+ sensing.Biomaterials · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The airway surface liquid (ASL) is the thin fluid layer lining airway surface epithelial cells, whose volume and composition are tightly regulated and may be abnormal in cystic fibrosis (CF). We synthesized a two-color fluorescent dextran to measure ASL [K(+)], TAC-Lime-dextran-TMR, consisting of a green-fluorescing triazacryptand K(+) ionophore-Bodipy conjugate, coupled to dextran, together with a red fluorescing tetramethylrhodamine reference chromophore. TAC-Lime-dextran-TMR fluorescence was K(+)-selective, increasing >4-fold with increasing [K(+)] from 0 to 40 mm. In well differentiated human airway epithelial cells, ASL [K(+)] was 20.8 +/- 0.3 mm and decreased by inhibition of the Na(+)/K(+) pump (ouabain), ENaC (amiloride), CF transmembrane conductance regulator (CFTR(inh)-172), or K(+) channels (TEA or XE991). ASL [K(+)] was increased by forskolin but not affected by Na(+)/K(+)/2Cl(-) cotransporter inhibition (bumetanide). Functional and expression studies indicated the involvement of [K(+)] channels KCNQ1, KCNQ3, and KCNQ5 as determinants of ASL [K(+)]. [K(+)] in CF cultures was similar to that in non-CF cultures, suggesting that abnormal ASL [K(+)] is not a factor in CF lung disease. In intact airways, ASL [K(+)] was also well above extracellular [K(+)]: 22 +/- 1 mm in pig trachea ex vivo and 16 +/- 1 mm in mouse trachea in vivo. Our results provide the first noninvasive measurements of [K(+)] in the ASL and indicate the involvement of apical and basolateral membrane ion transporters in maintaining a high ASL [K(+)].
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