ArticleNature methods2009
Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells.
Article in Nature methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
52 citing papers in PubMed, 161 citations in OpenAlex.
- An integrated nanophotonic SPRM microsystem for real-time, label-free quantification of single-cell adhesion dynamics.Microsystems & nanoengineering · 2026Article
- Mechanics and disease of heart cells/cardiomyocytes explored through atomic force microscopy: present and future.Biophysical reviews · 2025Review
- An innovative cellular medicine approach via the utilization of novel nanotechnology-based biomechatronic platforms as a label-free biomarker for early melanoma diagnosis.Scientific reports · 2024Article
- Side-view optical microscopy-assisted atomic force microscopy for thickness-dependent nanobiomechanics.Nanoscale advances · 2024Article
- Quantitative comparison of cell-cell detachment force in different experimental setups.The European physical journal. E, Soft matter · 2024Article
- Uniaxial Cyclic Cell Stretching Device for Accelerating Cellular Studies.Micromachines · 2023Article
- Recent advances in oblique plane microscopy.Nanophotonics (Berlin, Germany) · 2023Review
- Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.Progress in biophysics and molecular biology · 2022Review
- Optical Force of Bessel Pincer Light-Sheets Beam on a Dielectric Sphere of Arbitrary Size.Nanomaterials (Basel, Switzerland) · 2022Article
- Mechanical properties of external confinement modulate the rounding dynamics of cells.Biophysical journal · 2021Article
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- 3D mechanical characterization of single cells and small organisms using acoustic manipulation and force microscopy.Nature communications · 2021Article
- Bone fracture healing: perspectives according to molecular basis.Journal of bone and mineral metabolism · 2021Review
- Pulsed laser activated impulse response encoder (PLAIRE): sensitive evaluation of surface cellular stiffness on zebrafish embryos.Biomedical optics express · 2021Article
- Dynamic light scattering microrheology for soft and living materials.Soft matter · 2021Article
- Visually precise, low-damage, single-cell spatial manipulation with single-pixel resolution.Chemical science · 2021Article
- Stiffness of HIV-1 Mimicking Polymer Nanoparticles Modulates Ganglioside-Mediated Cellular Uptake and Trafficking.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- (De)form and Function: Measuring Cellular Forces with Deformable Materials and Deformable Structures.Advanced healthcare materials · 2020Review
- Quantifying single-platelet biomechanics: An outsider's guide to biophysical methods and recent advances.Research and practice in thrombosis and haemostasis · 2020Review
- Dynamic bonds and their roles in mechanosensing.Current opinion in chemical biology · 2019Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The mechanical rigidity of cells and adhesion forces between cells are important in various biological processes, including cell differentiation, proliferation and tissue organization. Atomic force microscopy has emerged as a powerful tool to quantify the mechanical properties of individual cells and adhesion forces between cells. Here we demonstrate an instrument that combines atomic force microscopy with a side-view fluorescent imaging path that enables direct imaging of cellular deformation and cytoskeletal rearrangements along the axis of loading. With this instrument, we directly observed cell shape under mechanical load, correlated changes in shape with force-induced ruptures and imaged formation of membrane tethers during cell-cell adhesion measurements. Additionally, we observed cytoskeletal reorganization and stress-fiber formation while measuring the contractile force of an individual cell. This instrument can be a useful tool for understanding the role of mechanics in biological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.