ArticleJournal of experimental botany2009
Complete blockage of the mevalonate pathway results in male gametophyte lethality.
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 82 citations in OpenAlex.
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- Insights into the Molecular Basis of Pollen Coat Development and Its Role in Male Sterility.International journal of molecular sciences · 2025Review
- Investigating the Role of Known Arabidopsis Iron Genes in a Stress Resilient Soybean Line.International journal of molecular sciences · 2024Article
- Overexpression of theFrontiers in plant science · 2024Article
- Arabidopsis 3β-Hydroxysteroid Dehydrogenases/C4-Decarboxylases Are Essential for the Pollen and Embryonic Development.International journal of molecular sciences · 2023Article
- Biosynthesis and transport of pollen coat precursors in angiosperms.Nature plants · 2023Review
- The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense againstInternational journal of molecular sciences · 2023Article
- Expressional diversity of grapevine 3-Hydroxy-3-methylglutaryl-CoA reductase (VvHMGR) in different grapes genotypes.BMC plant biology · 2021Article
- Metabolic and transcriptional analyses in response to potent inhibitors establish MEP pathway as major route for camptothecin biosynthesis in Nothapodytes nimmoniana (Graham) Mabb.BMC plant biology · 2019Article
- Changes in the expression of some genes involved in the biosynthesis of secondary metabolites in Cuminum cyminum L. under UV stress.Protoplasma · 2019Article
- The mevalonate pathway but not the methylerythritol phosphate pathway is critical for elaioplast and pollen coat development inPlant biotechnology (Tokyo, Japan) · 2018Article
- Evidence that thePlant biotechnology (Tokyo, Japan) · 2018Article
- A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice.Scientific reports · 2017Article
- Suppressing Farnesyl Diphosphate Synthase Alters Chloroplast Development and Triggers Sterol-Dependent Induction of Jasmonate- and Fe-Related Responses.Plant physiology · 2016Article
- Sterol Methyl Oxidases Affect Embryo Development via Auxin-Associated Mechanisms.Plant physiology · 2016Article
- Molecular characterization of HbCZF1, a Hevea brasiliensis CCCH-type zinc finger protein that regulates hmg1.Plant cell reports · 2015Article
- Plant Sterol Diversity in Pollen from Angiosperms.Lipids · 2015Article
- Functional analysis of 3-hydroxy-3-methylglutaryl coenzyme a reductase encoding genes in triterpene saponin-producing ginseng.Plant physiology · 2014Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants have two isoprenoid biosynthetic pathways: the cytosolic mevalonate (MVA) pathway and the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Since the discovery of the MEP pathway, possible metabolic cross-talk between these pathways has prompted intense research. Although many studies have shown the existence of such cross-talk using feeding experiments, it remains to be determined if native cross-talk, rather than exogenously applied metabolites, can compensate for complete blockage of the MVA pathway. Previously, Arabidopsis mutants for HMG1 and HMG2 encoding HMG-CoA reductase (HMGR) were isolated. Although it was shown that HMGR1 is a functional HMGR, the enzyme activity of HMGR2 has not been confirmed. It is demonstrated here that HMG2 encodes a functional reductase with similar activity to HMGR1, using enzyme assays and complementation experiments. To estimate the contribution of native cross-talk, an attempt was made to block the MVA pathway by making double mutants lacking both HMG1 and HMG2, but no double homozygotes were detected in the progeny of self-pollinated HMG1/hmg1 hmg2/hmg2 plants. hmg1 hmg2 male gametophytes appeared to be lethal based on crossing experiments, and microscopy indicated that approximately 50% of the microspores from the HMG1/hmg1 hmg2/hmg2 plant appeared shrunken and exhibited poorly defined endoplasmic reticulum membranes. In situ hybridization showed that HMG1 transcripts were expressed in both the tapetum and microspores, while HMG2 mRNA appeared only in microspores. It is concluded that native cross-talk from the plastid cannot compensate for complete blockage of the MVA pathway, at least during male gametophyte development, because either HMG1 or HMG2 is required for male gametophyte development.
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