ArticlePlant physiology2009
A nonsense mutation in a cinnamyl alcohol dehydrogenase gene is responsible for the Sorghum brown midrib6 phenotype.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 201 citations in OpenAlex.
- A NovelPlants (Basel, Switzerland) · 2026Article
- Brown midrib mutants in sorghum and their applications in renewable energy production.Frontiers in plant science · 2026Review
- From fibers to flowering to metabolites: unlocking hemp (Cannabis sativa) potential with the guidance of novel discoveries and tools.Journal of experimental botany · 2025Review
- Transcriptome andFrontiers in plant science · 2025Article
- Hyperspectral imaging to characterize the vegetative tissue biochemical changes in response to water deficit conditions in sorghum (Frontiers in plant science · 2025Article
- Decoding the genetic blueprint: regulation of key agricultural traits in sorghum.Advanced biotechnology · 2024Review
- Genome-wide characterization and expression analysis of the CINNAMYL ALCOHOL DEHYDROGENASE gene family in Triticum aestivum.BMC genomics · 2024Article
- Grass lignin: biosynthesis, biological roles, and industrial applications.Frontiers in plant science · 2024Review
- Genome-wide identification of theFrontiers in plant science · 2024Article
- Lignin, the Lignification Process, and Advanced, Lignin-Based Materials.International journal of molecular sciences · 2023Review
- A large-scale forward genetic screen for maize mutants with altered lignocellulosic properties.Frontiers in plant science · 2023Article
- Comparative transcriptome analysis of resistant and susceptible wheat in response to Rhizoctonia cerealis.BMC plant biology · 2022Article
- Comprehensive Analysis of Endogenous Volatile Compounds, Transcriptome, and Enzyme Activity RevealsFrontiers in plant science · 2022Article
- Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings.Scientific reports · 2021Article
- Pathogen and drought stress affect cell wall and phytohormone signaling to shape host responses in a sorghum COMT bmr12 mutant.BMC plant biology · 2021Article
- Cloning, expression analysis and molecular marker development of cinnamyl alcohol dehydrogenase gene in common wheat.Protoplasma · 2021Article
- Association of gene expression with syringyl to guaiacyl ratio in sugarcane lignin.Plant molecular biology · 2021Article
- SorghumGenes · 2021Article
- Genome-wide analysis of general phenylpropanoid and monolignol-specific metabolism genes in sugarcane.Functional & integrative genomics · 2021Article
- The Sorghum (Frontiers in plant science · 2021Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brown midrib6 (bmr6) affects phenylpropanoid metabolism, resulting in reduced lignin concentrations and altered lignin composition in sorghum (Sorghum bicolor). Recently, bmr6 plants were shown to have limited cinnamyl alcohol dehydrogenase activity (CAD; EC 1.1.1.195), the enzyme that catalyzes the conversion of hydroxycinnamoyl aldehydes (monolignals) to monolignols. A candidate gene approach was taken to identify Bmr6. Two CAD genes (Sb02g024190 and Sb04g005950) were identified in the sorghum genome based on similarity to known CAD genes and through DNA sequencing a nonsense mutation was discovered in Sb04g005950 that results in a truncated protein lacking the NADPH-binding and C-terminal catalytic domains. Immunoblotting confirmed that the Bmr6 protein was absent in protein extracts from bmr6 plants. Phylogenetic analysis indicated that Bmr6 is a member of an evolutionarily conserved group of CAD proteins, which function in lignin biosynthesis. In addition, Bmr6 is distinct from the other CAD-like proteins in sorghum, including SbCAD4 (Sb02g024190). Although both Bmr6 and SbCAD4 are expressed in sorghum internodes, an examination of enzymatic activity of recombinant Bmr6 and SbCAD4 showed that Bmr6 had 1 to 2 orders of magnitude greater activity for monolignol substrates. Modeling of Bmr6 and SbCAD4 protein structures showed differences in the amino acid composition of the active site that could explain the difference in enzyme activity. These differences include His-57, which is unique to Bmr6 and other grass CADs. In summary, Bmr6 encodes the major CAD protein involved in lignin synthesis in sorghum, and the bmr6 mutant is a null allele.
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