ArticleApplied and environmental microbiology2009
Detection of Toxoplasma gondii oocysts in water sample concentrates by real-time PCR.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Molecular Occurrence, Co-infections and Genetic Characterization of Sarcocystis miescheriana, Toxoplasma gondii and Neospora caninum in Wild Boars (Sus scrofa) from Türkiye.Acta parasitologica · 2026Article
- Strain-dependent effects of Toxoplasma gondii on ovarian health and inflammation in a rat model.BMC infectious diseases · 2025Article
- Molecular evidence ofItalian journal of food safety · 2024Article
- Comparative Evaluation of Re 529-Bp Sequence and B1 Gene in the Detection of Toxoplasma gondii Through PCR in Water Samples of Denizli, Turkey.Acta parasitologica · 2022Article
- Molecular Methods for the Detection ofMicroorganisms · 2021Review
- Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020Article
- Comparison of PCR assays to detect Toxoplasma gondii oocysts in green-lipped mussels (Perna canaliculus).Parasitology research · 2019Article
- Evaluation of propidium monoazide-based qPCR to detect viable oocysts of Toxoplasma gondii.Parasitology research · 2019Article
- Real-time PCR detection of Toxoplasma gondii in surface water samples in São Paulo, Brazil.Parasitology research · 2019Article
- Evaluating the presence of Toxoplasma gondii in peripheral blood of patients with diverse forms of uveitis.International ophthalmology · 2017Article
- Molecules to modeling: Toxoplasma gondii oocysts at the human-animal-environment interface.Comparative immunology, microbiology and infectious diseases · 2013Review
- The impact of the waterborne transmission of Toxoplasma gondii and analysis efforts for water detection: an overview and update.Environmental science and pollution research international · 2013Review
- Loop-mediated isothermal amplification (LAMP): early detection of Toxoplasma gondii infection in mice.Parasites & vectors · 2012Article
- Determining UV inactivation of Toxoplasma gondii oocysts by using cell culture and a mouse bioassay.Applied and environmental microbiology · 2010Article
- Bacterial Degraders of Coexisting Dichloromethane, Benzene, and Toluene, Identified by Stable-Isotope Probing.Water, air, and soil pollutionArticle
Corrections and comments
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Authors and funding
9 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR techniques in combination with conventional parasite concentration procedures have potential for the sensitive and specific detection of Toxoplasma gondii oocysts in water. Three real-time PCR assays based on the B1 gene and a 529-bp repetitive element were analyzed for the detection of T. gondii tachyzoites and oocysts. Lower sensitivity and specificity were obtained with the B1 gene-based PCR than with the 529-bp repeat-based PCR. New procedures for the real-time PCR detection of T. gondii oocysts in concentrates of surface water were developed and tested in conjunction with a method for the direct extraction of inhibitor-free DNA from water. This technique detected as few as one oocyst seeded to 0.5 ml of packed pellets from water samples concentrated by Envirocheck filters. Thus, this real-time PCR may provide a detection method alternative to the traditional mouse assay and microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.