ArticleApplied and environmental microbiology2009
Viability PCR, a culture-independent method for rapid and selective quantification of viable Legionella pneumophila cells in environmental water samples.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 151 citations in OpenAlex.
- Comparative evaluation and validation of rapid quantification methods for Mycoplasma hyopneumoniae: development of a PMA-based viability qPCR assay.Scientific reports · 2026Article
- Contamination of liposomal bupivacaine during multi-dose usage in a clinical setting evaluated by culture and PCR.Frontiers in veterinary science · 2024Article
- A Pilot Study forSensors (Basel, Switzerland) · 2023Article
- Detection and quantification of viable but non-culturableFrontiers in microbiology · 2023Article
- Nanomaterials meet surface-enhanced Raman scattering towards enhanced clinical diagnosis: a review.Journal of nanobiotechnology · 2022Review
- Insights into microbial diversity on plastisphere by multi-omics.Archives of microbiology · 2022Review
- Confirming the Presence of Legionella pneumophila in Your Water System: A Review of Current Legionella Testing Methods.Journal of AOAC International · 2021Review
- Environmental Management ofMicroorganisms · 2021Review
- Legionnaires' Disease on the Rise in Switzerland: A Denominator-Based Analysis of National Diagnostic Data, 2007-2016.International journal of environmental research and public health · 2020Article
- Water as a Source of Antimicrobial Resistance and Healthcare-Associated Infections.Pathogens (Basel, Switzerland) · 2020Review
- Direct Metatranscriptome RNA-seq and Multiplex RT-PCR Amplicon Sequencing on Nanopore MinION - Promising Strategies for Multiplex Identification of Viable Pathogens in Food.Frontiers in microbiology · 2020Article
- On-filter direct amplification of Legionella pneumophila for rapid assessment of its abundance and viability.Water research · 2017Article
- Multiplex polymerase chain reaction of genetic markers for detection of potentially pathogenic environmentalThe Indian journal of medical research · 2017Article
- Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.Water research · 2017Review
- Viability of Legionella pneumophila in Water Samples: A Comparison of Propidium Monoazide (PMA) Treatment on Membrane Filters and in Liquid.International journal of environmental research and public health · 2017Article
- Most probable number - loop mediated isothermal amplification (MPN-LAMP) for quantifying waterborne pathogens in <25min.Journal of microbiological methods · 2017Article
- Necessity of purification during bacterial DNA extraction with environmental soils.Environmental health and toxicology · 2017Article
- Detection of Legionella Pneumophila in Urine and Serum Specimens of Neutropenic Febrile Patients with Haematological Malignancies.International journal of hematology-oncology and stem cell research · 2017Article
- Legionella Risk Management and Control in Potable Water Systems: Argument for the Abolishment of Routine Testing.International journal of environmental research and public health · 2016Article
- Comparative analysis of solar pasteurization versus solar disinfection for the treatment of harvested rainwater.BMC microbiology · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR-based methods have been developed to rapidly screen for Legionella pneumophila in water as an alternative to time-consuming culture techniques. However, these methods fail to discriminate between live and dead bacteria. Here, we report a viability assay (viability PCR [v-PCR]) for L. pneumophila that combines ethidium monoazide bromide with quantitative real-time PCR (qPCR). The ability of v-PCR to differentiate viable from nonviable L. pneumophila cells was confirmed with permeabilizing agents, toluene, or isopropanol. v-PCR suppressed more than 99.9% of the L. pneumophila PCR signal in nonviable cultures and was able to discriminate viable cells in mixed samples. A wide range of physiological states, from culturable to dead cells, was observed with 64 domestic hot-water samples after simultaneous quantification of L. pneumophila cells by v-PCR, conventional qPCR, and culture methods. v-PCR counts were equal to or higher than those obtained by culture and lower than or equal to conventional qPCR counts. v-PCR was used to successfully monitor in vitro the disinfection efficacy of heating to 70 degrees C and glutaraldehyde and chlorine curative treatments. The v-PCR method appears to be a promising and rapid technique for enumerating L. pneumophila bacteria in water and, in comparison with conventional qPCR techniques used to monitor Legionella, has the advantage of selectively amplifying only viable cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.