ArticleApplied and environmental microbiology2009
Characterization of a unique class C acid phosphatase from Clostridium perfringens.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Thermotolerant class A acid phosphatase active across broad pH range and diverse substrates.Protein science : a publication of the Protein Society · 2025Article
- Characterization of an extremophile bacterial acid phosphatase derived from metagenomics analysis.Microbial biotechnology · 2024Article
- Detection and expression of SapS, a class C nonspecific acid phosphatase with O-phospho-Ltyrosine- phosphatase activity, in Staphylococcus aureus isolates from patients with chronic osteomyelitisBiomedica : revista del Instituto Nacional de Salud · 2023Article
- Characterization of the Secreted Acid Phosphatase SapS Reveals a Novel Virulence Factor ofInternational journal of molecular sciences · 2022Article
- Isolation and characterization of a recombinant class C acid phosphatase fromBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- Microbial 5'-nucleotidases: their characteristics, roles in cellular metabolism, and possible practical applications.Applied microbiology and biotechnology · 2021Review
- Biochemical characterization of a recombinant acid phosphatase from Acinetobacter baumannii.PloS one · 2021Article
- Bacteria from the endosphere and rhizosphere of Quercus spp. use mainly cell wall-associated enzymes to decompose organic matter.PloS one · 2019Article
- Phylogenetic distribution, biogeography and the effects of land management upon bacterial non-specific Acid phosphatase Gene diversity and abundance.Plant and soil · 2018Article
- Structural basis of the inhibition of class C acid phosphatases by adenosine 5'-phosphorothioate.The FEBS journal · 2011Article
- Expression, purification and crystallization of an atypical class C acid phosphatase from Mycoplasma bovis.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Crystal structure and immunogenicity of the class C acid phosphatase from Pasteurella multocida.Archives of biochemistry and biophysics · 2011Article
- Recognition of nucleoside monophosphate substrates by Haemophilus influenzae class C acid phosphatase.Journal of molecular biology · 2010Article
- Expression, purification and crystallization of class C acid phosphatases from Francisella tularensis and Pasteurella multocida.Acta crystallographica. Section F, Structural biology and crystallization communications · 2009Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium perfringens is a gram-positive anaerobe and a pathogen of medical importance. The detection of acid phosphatase activity is a powerful diagnostic indicator of the presence of C. perfringens among anaerobic isolates; however, characterization of the enzyme has not previously been reported. Provided here are details of the characterization of a soluble recombinant form of this cell-associated enzyme. The denatured enzyme was approximately 31 kDa and a homodimer in solution. It catalyzed the hydrolysis of several substrates, including para-nitrophenyl phosphate, 4-methylumbelliferyl phosphate, and 3' and 5' nucleoside monophosphates at pH 6. Calculated K(m)s ranged from 0.2 to 0.6 mM with maximum velocity ranging from 0.8 to 1.6 micromol of P(i)/s/mg. Activity was enhanced in the presence of some divalent cations but diminished in the presence of others. Wild-type enzyme was detected in all clinical C. perfringens isolates tested and found to be cell associated. The described enzyme belongs to nonspecific acid phosphatase class C but is devoid of lipid modification commonly attributed to this class.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.