ArticleApplied and environmental microbiology2009
High-throughput quantitative analysis of the human intestinal microbiota with a phylogenetic microarray.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed.
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- QIIME2 enhances multi-amplicon sequencing data analysis: a standardized and validated open-source pipeline for comprehensive 16S rRNA gene profiling.Microbiology spectrum · 2025Article
- Microbial Community Composition Associated with Potato Plants Displaying Early Dying Syndrome.Microorganisms · 2025Article
- Diversity and Vertical Distribution of Planctomycetota in the Water Column of the Remote North Pacific.Environmental microbiology reports · 2025Article
- Correlation between the gut microbiome and neurodegenerative diseases: a review of metagenomics evidence.Neural regeneration research · 2024Review
- Novel Techniques and Models for Studying the Role of the Gut Microbiota in Drug Metabolism.European journal of drug metabolism and pharmacokinetics · 2024Review
- Article
- Gut Microbiota Changes and Its Potential Relations with Thyroid Disorders: From Composition to Therapeutic Targets.International journal of general medicine · 2024Review
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- Mechanistic Insights into Immune-Microbiota Interactions and Preventive Role of Probiotics Against Autoimmune Diabetes Mellitus.Probiotics and antimicrobial proteins · 2023Review
- Long-read sequencing reveals the shell microbiome of apparently healthy American lobstersFrontiers in microbiology · 2023Article
- Diet-Host-Microbiota Interactions Shape Aryl Hydrocarbon Receptor Ligand Production to Modulate Intestinal Homeostasis.Annual review of nutrition · 2021Review
- Gut microbiota: effect of pubertal status.BMC microbiology · 2020Article
- Is adolescence the missing developmental link in Microbiome-Gut-Brain axis communication?Developmental psychobiology · 2019Review
- Gut-microbiota-on-a-chip: an enabling field for physiological research.Microphysiological systems · 2018Article
- Mechanisms of utilisation of arabinoxylans by a porcine faecal inoculum: competition and co-operation.Scientific reports · 2018Article
- New Frontiers in Genetics, Gut Microbiota, and Immunity: A Rosetta Stone for the Pathogenesis of Inflammatory Bowel Disease.BioMed research international · 2017Review
- Ultrafiltration and Microarray for Detection of Microbial Source Tracking Marker and Pathogen Genes in Riverine and Marine Systems.Applied and environmental microbiology · 2016Article
- Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism ofFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gut microbiota carry out key functions in health and participate in the pathogenesis of a growing number of diseases. The aim of this study was to develop a custom microarray that is able to identify hundreds of intestinal bacterial species. We used the Entrez nucleotide database to compile a data set of bacterial 16S rRNA gene sequences isolated from human intestinal and fecal samples. Identified sequences were clustered into separate phylospecies groups. Representative sequences from each phylospecies were used to develop a microbiota microarray based on the Affymetrix GeneChip platform. The designed microbiota array contains probes to 775 different bacterial phylospecies. In our validation experiments, the array correctly identified genomic DNA from all 15 bacterial species used. Microbiota array has a detection sensitivity of at least 1 pg of genomic DNA and can detect bacteria present at a 0.00025% level of overall sample. Using the developed microarray, fecal samples from two healthy children and two healthy adults were analyzed for bacterial presence. Between 227 and 232 species were detected in fecal samples from children, whereas 191 to 208 species were found in adult stools. The majority of identified phylospecies belonged to the classes Clostridia and Bacteroidetes. The microarray revealed putative differences between the gut microbiota of healthy children and adults: fecal samples from adults had more Clostridia and less Bacteroidetes and Proteobacteria than those from children. A number of other putative differences were found at the genus level.
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Registered trials
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