ArticleApplied and environmental microbiology2009
Recovery and detection of Escherichia coli O157:H7 in surface water, using ultrafiltration and real-time PCR.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- First Reported Outbreak of Persistent Shiga Toxin-producingOpen forum infectious diseases · 2026Article
- An investigation of an outbreak ofFood control · 2024Article
- UV-Vis detection ofMethodsX · 2024Article
- Shiga Toxin-Producing Escherichia coli O157:H7 Illness Outbreak Associated with Untreated, Pressurized, Municipal Irrigation Water - Utah, 2023.MMWR. Morbidity and mortality weekly report · 2024Article
- An Outbreak Investigation of Salmonella Typhimurium Illnesses in the United States Linked to Packaged Leafy Greens Produced at a Controlled Environment Agriculture Indoor Hydroponic Operation - 2021.Journal of food protection · 2023Article
- The Persistence of Bacterial Pathogens in Surface Water and Its Impact on Global Food Safety.Pathogens (Basel, Switzerland) · 2021Review
- Separation of Escherichia coli from natural samples for identification of sources and microcosm inoculation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- An automated bacterial concentration and recovery system for pre-enrichment required in rapid Escherichia coli detection.Scientific reports · 2018Article
- A review on detection methods used for foodborne pathogens.The Indian journal of medical research · 2016Review
- Article
- Microbial and chemical contamination during and after flooding in the Ohio River-Kentucky, 2011.Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering · 2014Article
- Microbiological quality of fresh produce from open air markets and supermarkets in the Philippines.TheScientificWorldJournal · 2014Article
- Recovery of diverse microbes in high turbidity surface water samples using dead-end ultrafiltration.Journal of microbiological methods · 2012Article
- Improving Salmonella determination in Sinaloa rivers with ultrafiltration and most probable number methods.Environmental monitoring and assessment · 2012Article
- Development of a PCR protocol for the detection of Escherichia coli O157:H7 and Salmonella spp. in surface water.Environmental monitoring and assessment · 2011Article
- Tangential-flow ultrafiltration with integrated inhibition detection for recovery of surrogates and human pathogens from large-volume source water and finished drinking water.Applied and environmental microbiology · 2011Article
- Enrichment and detection of Escherichia coli O157:H7 from water samples using an antibody modified microfluidic chip.Analytical chemistry · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterohemorrhagic Escherichia coli O157:H7 (EHEC O157:H7) outbreaks have revealed the need for improved analytical techniques for environmental samples. Ultrafiltration (UF) is increasingly recognized as an effective procedure for concentrating and recovering microbes from large volumes of water and treated wastewater. This study describes the application of hollow-fiber UF as the primary step for concentrating EHEC O157:H7 seeded into 40-liter samples of surface water, followed by an established culture/immunomagnetic-separation (IMS) method and a suite of real-time PCR assays. Three TaqMan assays were used to detect the stx1, stx2, and rfbE gene targets. The results from this study indicate that approximately 50 EHEC O157:H7 cells can be consistently recovered from a 40-liter surface water sample and detected by culture and real-time PCR. Centrifugation was investigated and shown to be a viable alternative to membrane filtration in the secondary culture/IMS step when water quality limits the volume of water that can be processed by a filter. Using multiple PCR assay sets to detect rfbE, stx1, and stx2 genes allowed for specific detection of EHEC O157:H7 from strains that do not possess all three genes. The reported sample collection and analysis procedure should be a sensitive and effective tool for detecting EHEC O157:H7 in response to outbreaks of disease associated with contaminated water.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.