ArticleMethods in enzymology2009
Type I polyketide synthases that require discrete acyltransferases.
Article in Methods in enzymology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Modular biosynthesis of microbiome-derived polyketides and nonribosomal peptides: insights and opportunities for Lactobacillaceae.Microbial cell factories · 2026Review
- Research progress of glutarimide-containing polyketides: Structures, bioactivities and their biosynthesis.Pharmaceutical science advances · 2025Review
- Bongkrekic Acid andFoods (Basel, Switzerland) · 2023Review
- Crosstalk between primary and secondary metabolism: Interconnected fatty acid and polyketide biosynthesis in prokaryotes.Bioorganic & medicinal chemistry letters · 2023Review
- Fungal BGCs for Production of Secondary Metabolites: Main Types, Central Roles in Strain Improvement, and Regulation According to the Piano Principle.International journal of molecular sciences · 2023Review
- Biosynthesis of Largimycins inACS chemical biology · 2022Article
- An Unconventional Melanin Biosynthesis Pathway in Ustilago maydis.Applied and environmental microbiology · 2021Article
- Biosynthesis Gene Cluster and Oxazole Ring Formation Enzyme for Inthomycins inApplied and environmental microbiology · 2020Article
- Conjugative Transposons and Their Cargo Genes Vary across Natural Populations of Rickettsia buchneri Infecting the Tick Ixodes scapularis.Genome biology and evolution · 2018Article
- Acyltransferases as Tools for Polyketide Synthase Engineering.Antibiotics (Basel, Switzerland) · 2018Review
- Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis.PloS one · 2018Article
- A Protein Interaction Map of the Kalimantacin Biosynthesis Assembly Line.Frontiers in microbiology · 2016Article
- Structural and evolutionary relationships of "AT-less" type I polyketide synthase ketosynthases.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Genetic determinants of reutericyclin biosynthesis in Lactobacillus reuteri.Applied and environmental microbiology · 2015Article
- Article
- Cycloheximide and actiphenol production in Streptomyces sp. YIM56141 governed by single biosynthetic machinery featuring an acyltransferase-less type I polyketide synthase.Organic letters · 2014Article
- Post-polyketide synthase steps in iso-migrastatin biosynthesis, featuring tailoring enzymes with broad substrate specificity.Journal of the American Chemical Society · 2013Article
- Crystallization and preliminary X-ray analysis of the bacillaene synthase trans-acting acyltransferase PksC.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Reconstitution of the FK228 biosynthetic pathway reveals cross talk between modular polyketide synthases and fatty acid synthase.Applied and environmental microbiology · 2011Article
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4 authors at 1 institution in 1 country.
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Abstract
The diverse structures of polyketide natural products are reflected by the equally diverse polyketide biosynthetic enzymes, namely polyketide synthases (PKSs). Three major classes of PKSs are known-noniterative type I PKSs, iterative type II PKSs and acyl carrier protein-independent type III PKSs, each of which consists of additional variants. One such variant is the noniterative type I PKS in which each PKS module lacks the cognate acyltransferase (AT) domain. The essential AT activity is instead provided by a discrete AT in trans. Termed "AT-less" type I PKSs, the loading of the malonate extender units by the discrete AT enzyme LnmG to each of the AT-less PKS modules of LnmI and LnmJ was confirmed experimentally for biosynthesis of the anticancer antibiotic leinamycin (LNM). The LNM PKS has since served as a model for the continuous discovery of numerous additional AT-less type I PKSs incorporating variable extender units. However, biochemical characterization of AT-less type I PKSs remains very limited, and the mechanism by which AT-less type I PKSs accommodate multiple extender units is unknown. This chapter provides the protocols used to establish and characterize the LNM PKS. Application of these methods to other AT-less type I PKSs should aid the biochemical characterization and hence possible exploitation of these unique PKSs for polyketide natural product structural diversity by combinatorial biosynthetic methods.
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