ArticleJournal of molecular biology2009
Kinetic analysis of the guanine nucleotide exchange activity of TRAPP, a multimeric Ypt1p exchange factor.
Article in Journal of molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 25 citations in OpenAlex.
- TRAPPC6B biallelic variants cause a neurodevelopmental disorder with TRAPP II and trafficking disruptions.Brain : a journal of neurology · 2024Article
- The TRAPP complexes: oligomeric exchange factors that activate the small GTPases Rab1 and Rab11.FEBS letters · 2023Review
- The substrate specificity of the human TRAPPII complex's Rab-guanine nucleotide exchange factor activity.Communications biology · 2020Article
- Ragulator and SLC38A9 activate the Rag GTPases through noncanonical GEF mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Nup159 Weakens Gle1 Binding to Dbp5 But Does Not Accelerate ADP Release.Journal of molecular biology · 2018Article
- Molecular control of Rab activity by GEFs, GAPs and GDI.Small GTPases · 2018Review
- Rab GTPases and their interacting protein partners: Structural insights into Rab functional diversity.Small GTPases · 2018Review
- P(I) Release Limits the Intrinsic and RNA-Stimulated ATPase Cycles of DEAD-Box Protein 5 (Dbp5).Journal of molecular biology · 2016Article
- Article
- Rab GTPase regulation of membrane identity.Current opinion in cell biology · 2013Review
- Take advantage of time in your experiments: a guide to simple, informative kinetics assays.Molecular biology of the cell · 2013Article
- Quantitative Analysis of Guanine Nucleotide Exchange Factors (GEFs) as Enzymes.Cellular logistics · 2013Article
- Retrograde vesicle transport in the Golgi.Protoplasma · 2012Review
- Nucleotide exchange factors: Kinetic analyses and the rationale for studying kinetics of GEFs.Cellular logistics · 2012Article
- ATP utilization and RNA conformational rearrangement by DEAD-box proteins.Annual review of biophysics · 2012Review
- Insights regarding guanine nucleotide exchange from the structure of a DENN-domain protein complexed with its Rab GTPase substrate.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Mechanism of Mss116 ATPase reveals functional diversity of DEAD-Box proteins.Journal of molecular biology · 2011Article
- Article
- Rab GEFs and GAPs.Current opinion in cell biology · 2010Review
- Structural mechanisms for regulation of membrane traffic by rab GTPases.Traffic (Copenhagen, Denmark) · 2009Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
TRAPP complexes, which are large multimeric assemblies that function in membrane traffic, are guanine nucleotide exchange factors (GEFs) that activate the Rab GTPase Ypt1p. Here we measured rate and equilibrium constants that define the interaction of Ypt1p with guanine nucleotide (guanosine 5'-diphosphate and guanosine 5'-triphosphate/guanosine 5'-(beta,gamma-imido)triphosphate) and the core TRAPP subunits required for GEF activity. These parameters allowed us to identify the kinetic and thermodynamic bases by which TRAPP catalyzes nucleotide exchange from Ypt1p. Nucleotide dissociation from Ypt1p is slow (approximately 10(-4) s(-1)) and accelerated >1000-fold by TRAPP. Acceleration of nucleotide exchange by TRAPP occurs via a predominantly Mg(2+)-independent pathway. Thermodynamic linkage analysis indicates that TRAPP weakens nucleotide affinity by <80-fold and vice versa, in contrast to most other characterized GEF systems that weaken nucleotide binding affinities by 4-6 orders of magnitude. The overall net changes in nucleotide binding affinities are small because TRAPP accelerates both nucleotide binding and dissociation from Ypt1p. Weak thermodynamic coupling allows TRAPP, Ypt1p, and nucleotide to exist as a stable ternary complex, analogous to strain-sensing cytoskeleton motors. These results illustrate a novel strategy of guanine nucleotide exchange by TRAPP that is particularly suited for a multifunctional GEF involved in membrane traffic.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.