ArticleDevelopmental biology2009
The C. elegans sex determination gene laf-1 encodes a putative DEAD-box RNA helicase.
Article in Developmental biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Heat stress promotes GTBP-1 association with perinuclear P-bodies in the C. elegans germline.PLoS genetics · 2026Article
- Evolutionary changes of noncoding elements associated with transition of sexual mode inScience advances · 2024Article
- Identification of BiP as a temperature sensor mediating temperature-induced germline sex reversal in C. elegans.The EMBO journal · 2024Article
- ATP-induced cross-linking of a biomolecular condensate.Biophysical journal · 2024Article
- ADR-2 regulates fertility and oocyte fate inbioRxiv : the preprint server for biology · 2024Article
- ATP-induced crosslinking of a biomolecular condensate.bioRxiv : the preprint server for biology · 2023Article
- Review
- Regulatory roles ofFrontiers in physiology · 2022Article
- Sex Determination in Nematode Germ Cells.Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation · 2022Review
- Identifying sequence perturbations to an intrinsically disordered protein that determine its phase-separation behavior.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Cloning and expression analysis of the DEAD-box/RNA helicase Oslaf-1 in Ovomermis sinensis.PloS one · 2018Article
- RNAi Screen Identifies Novel Regulators of RNP Granules in the Caenorhabditis elegans Germ Line.G3 (Bethesda, Md.) · 2016Article
- The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The DEAD Box RNA helicase VBH-1 is a new player in the stress response in C. elegans.PloS one · 2014Article
- The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes.Developmental biology · 2011Article
- Somatic sex determination in Caenorhabditis elegans is modulated by SUP-26 repression of tra-2 translation.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Bioinformatic analysis of P granule-related proteins: insights into germ granule evolution in nematodes.Development genes and evolution · 2010Article
- Article
- P granule assembly and function in Caenorhabditis elegans germ cells.Journal of andrologyReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Caenorhabditis elegans gene laf-1 is critical for both embryonic development and sex determination. Laf-1 is thought to promote male cell fates by negatively regulating expression of tra-2 in both hermaphrodites and males. We cloned laf-1 and established that it encodes a putative DEAD-box RNA helicase related to Saccharomycescerevisiae Ded1p and Drosophila Vasa. Three sequenced laf-1 mutations are missense alleles affecting a small region of the protein in or near helicase motif III. We demonstrate that the phenotypes resulting from laf-1 mutations are due to loss or reduction of laf-1 function, and that both laf-1 and a related helicase vbh-1 function in germline sex determination. Laf-1 mRNA is expressed in both males and hermaphrodites and in both the germline and soma of hermaphrodites. It is expressed at all developmental stages and is most abundant in embryos. LAF-1 is predominantly, if not exclusively, cytoplasmic and colocalizes with PGL-1 in P granules of germline precursor cells. Previous results suggest that laf-1 functions to negatively regulate expression of the sex determination protein TRA-2, and we find that the abundance of TRA-2 is modestly elevated in laf-1/+ females. We discuss potential functions of LAF-1 as a helicase and its roles in sex determination.
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