ArticleJournal of molecular biology2009
Oligomeric structure and functional characterization of the urea transporter from Actinobacillus pleuropneumoniae.
Article in Journal of molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Microbial conversion of biodiesel waste for carotenoid production.Frontiers in bioengineering and biotechnology · 2026Article
- Protein Structures of Urea Transporters.Sub-cellular biochemistry · 2025Review
- Genomic insight into strategy, interaction and evolution of nitrifiers in metabolizing key labile-dissolved organic nitrogen in different environmental niches.Frontiers in microbiology · 2023Article
- Urea-aromatic interactions in biology.Biophysical reviews · 2020Review
- Aestivation Induces Changes in the mRNA Expression Levels and Protein Abundance of Two Isoforms of Urea Transporters in the Gills of the African Lungfish,Frontiers in physiology · 2017Article
- Structure of urea transporters.Sub-cellular biochemistry · 2014Review
- Lipid, detergent, and Coomassie Blue G-250 affect the migration of small membrane proteins in blue native gels: mitochondrial carriers migrate as monomers not dimers.The Journal of biological chemistry · 2013Article
- Mechanisms of molecular transport through the urea channel of Helicobacter pylori.Nature communications · 2013Article
- Structure and permeation mechanism of a mammalian urea transporter.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The emerging physiological roles of the SLC14A family of urea transporters.British journal of pharmacology · 2011Review
- Transport kinetics and selectivity of HpUreI, the urea channel from Helicobacter pylori.Biochemistry · 2011Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Urea transporters (UTs) facilitate urea permeation across cell membranes in prokaryotes and eukaryotes. Bacteria use urea as a means to survive in acidic environments and/or as a nitrogen source. The UT from Actinobacillus pleuropneumoniae, ApUT, the pathogen that causes porcine pleurisy and pneumonia, was expressed in Escherichia coli and purified. Analysis of the recombinant protein using cross-linking and blue-native gel electrophoresis established that ApUT is a dimer in detergent solution. Purified protein was reconstituted into proteoliposomes and urea efflux was measured by stopped-flow fluorometry to determine the urea transport kinetics of ApUT. The measured urea flux was saturable, could be inhibited by phloretin, and was not affected by pH. Two-dimensional crystals of the biologically active ApUT show that it is also dimeric in a lipid membrane and provide the first structural information on a member of the UT family.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.