ArticleNature protocols2009
A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 147 citations in OpenAlex.
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- Chelation-based iron uptake mitigates the effects of prolonged high-temperature stress in cool-season grasses.Nature communications · 2025Article
- Transcription factor VRT2 reinitiates vernalization when interrupted by warm temperatures in a temperate grass model.Plant physiology · 2024Article
- Genome-wide identification of the plant homeodomain-finger family in rye and ScPHD5 functions in cold tolerance and flowering time.Plant cell reports · 2024Article
- Phytochromes transmit photoperiod information via the evening complex in Brachypodium.Genome biology · 2023Article
- VERNALIZATION1 represses FLOWERING PROMOTING FACTOR1-LIKE1 in leaves for timely flowering in Brachypodium distachyon.The Plant cell · 2023Article
- Fast-track transformation and genome editing in Brachypodium distachyon.Plant methods · 2023Article
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- A phosphorus-limitation induced, functionally conserved DUF506 protein is a repressor of root hair elongation in plants.The New phytologist · 2022Article
- Establishment of an efficientMolecular breeding : new strategies in plant improvement · 2021Article
- Changes in the Cell Wall Proteome of Leaves in Response to High Temperature Stress inInternational journal of molecular sciences · 2021Article
- Wheat FRIZZY PANICLE activates VERNALIZATION1-A and HOMEOBOX4-A to regulate spike development in wheat.Plant biotechnology journal · 2021Article
- Suppression of Phytochrome-Interacting Factors Enhances Photoresponses of Seedlings and Delays Flowering With Increased Plant Height inFrontiers in plant science · 2021Article
- Overexpression of a Cytochrome P450 Monooxygenase Involved in Orobanchol Biosynthesis Increases Susceptibility to Fusarium Head Blight.Frontiers in plant science · 2021Article
- BdWRKY38 is required for the incompatible interaction of Brachypodium distachyon with the necrotrophic fungus Rhizoctonia solani.The Plant journal : for cell and molecular biology · 2020Article
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- An improved protocol for efficient transformation and regeneration of Setaria italica.Plant cell reports · 2020Article
- Molecular Dissection of TaLTP1 Promoter Reveals Functional Cis-Elements Regulating Epidermis-Specific Expression.International journal of molecular sciences · 2020Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Brachypodium distachyon is a novel model system for structural and functional genomics studies of temperate grasses because of its biological and genetic attributes. Recently, the genome sequence of the community standard line Bd21 has been released and the availability of an efficient transformation system is critical for the discovery and validation of the function of Brachypodium genes. Here, we provide an improved procedure for the facile and efficient Agrobacterium-mediated transformation of line Bd21. The protocol relies on the transformation of compact embryogenic calli derived from immature embryos using visual and chemical screening of transformed tissues and plants. The combination of green fluorescent protein expression and hygromycin resistance enables early identification of transformation events and drastically reduces the quantity of tissue to be handled throughout the selection process. Approximately eight independent fully developed transgenic Bd21 plants can be produced from each immature embryo, enabling the generation of thousands of T-DNA lines. The process--from wild-type seeds to transgenic T(1) seeds--takes approximately 8 months to complete.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.