ArticleNucleic acids research2009
Rapid Cdc13 turnover and telomere length homeostasis are controlled by Cdk1-mediated phosphorylation of Cdc13.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- Set1 regulates telomere function via H3K4 methylation-dependent and -independent pathways and calibrates the abundance of telomere maintenance factors.Molecular biology of the cell · 2023Article
- Telomeres and Cancer.Life (Basel, Switzerland) · 2021Review
- The mechanisms of K. lactis Cdc13 in telomere DNA-binding and telomerase regulation.DNA repair · 2018Article
- Yeast Cip1 is activated by environmental stress to inhibit Cdk1-G1 cyclins via Mcm1 and Msn2/4.Nature communications · 2017Article
- The mre11 A470 alleles influence the hereditability and the segregation of telosomes in Saccharomyces cerevisiae.PloS one · 2017Article
- Structure and function of the telomeric CST complex.Computational and structural biotechnology journal · 2016Review
- Multiple facets of TPP1 in telomere maintenance.Biochimica et biophysica acta · 2014Review
- Synthetic cytotoxicity: digenic interactions with TEL1/ATM mutations reveal sensitivity to low doses of camptothecin.Genetics · 2014Article
- Global analysis of cdc14 dephosphorylation sites reveals essential regulatory role in mitosis and cytokinesis.Molecular & cellular proteomics : MCP · 2014Article
- Cdk1 regulates the temporal recruitment of telomerase and Cdc13-Stn1-Ten1 complex for telomere replication.Molecular and cellular biology · 2014Article
- Human TEN1 maintains telomere integrity and functions in genome-wide replication restart.The Journal of biological chemistry · 2013Article
- Phosphorylation of TPP1 regulates cell cycle-dependent telomerase recruitment.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Finding the end: recruitment of telomerase to telomeres.Nature reviews. Molecular cell biology · 2013Review
- Telomerase-dependent generation of 70-nt-long telomeric single-stranded 3' overhangs in yeast.Nucleic acids research · 2013Article
- Novel phosphorylation sites in the S. cerevisiae Cdc13 protein reveal new targets for telomere length regulation.Journal of proteome research · 2013Article
- An S/T-Q cluster domain census unveils new putative targets under Tel1/Mec1 control.BMC genomics · 2012Article
- CDK1 differentially regulates G-overhang generation at leading- and lagging-strand telomeres in telomerase-negative cells in G2 phase.Cell cycle (Georgetown, Tex.) · 2012Article
- The Cdc48 protein and its cofactor Vms1 are involved in Cdc13 protein degradation.The Journal of biological chemistry · 2012Article
- SUMOylation regulates telomere length homeostasis by targeting Cdc13.Nature structural & molecular biology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Budding yeast telomerase is mainly activated by Tel1/Mec1 (yeast ATM/ATR) on Cdc13 from late S to G2 phase of the cell cycle. Here, we demonstrated that the telomerase-recruitment domain of Cdc13 is also phosphorylated by Cdk1 at the same cell cycle stage as the Tel1/Mec1-dependent regulation. Phosphor-specific gel analysis demonstrated that Cdk1 phosphorylates residues 308 and 336 of Cdc13. The residue T308 of Cdc13 is critical for efficient Mec1-mediated S306 phosphorylation in vitro. Phenotypic analysis in vivo revealed that the mutations in the Cdc13 S/TP motifs phosphorylated by Cdk1 caused cell cycle delay and telomere shortening and these phenotypes could be partially restored by the replacement with a negative charge residue. In the absence of Ku or Tel1, Cdk1-mediated phosphorylation of Cdc13 showed no effect on telomere length maintenance. Moreover, this Cdk1-mediated phosphorylation was required to promote the regular turnover of Cdc13. Together these results demonstrate that Cdk1 phosphorylates the telomerase recruitment domain of Cdc13, thereby preserves optimal function and expression level of Cdc13 for precise telomere replication and cell cycle progression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.