ArticleThe EMBO journal1991
Intramolecular base pairing between the nematode spliced leader and its 5' splice site is not essential for trans-splicing in vitro.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
- On the Possibility of an Early Evolutionary Origin for the Spliced Leader Trans-Splicing.Journal of molecular evolution · 2017Review
- Analysis of C. elegans muscle transcriptome using trans-splicing-based RNA tagging (SRT).Nucleic acids research · 2016Article
- Polycistronic pre-mRNA processing in vitro: snRNP and pre-mRNA role reversal in trans-splicing.Genes & development · 2010Article
- The nematode eukaryotic translation initiation factor 4E/G complex works with a trans-spliced leader stem-loop to enable efficient translation of trimethylguanosine-capped RNAs.Molecular and cellular biology · 2010Article
- Exportin 1 mediates nuclear export of the kinetoplastid spliced leader RNA.Eukaryotic cell · 2003Article
- Trans-spliced leader addition to mRNAs in a cnidarian.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- trans splicing of polycistronic Caenorhabditis elegans pre-mRNAs: analysis of the SL2 RNA.Molecular and cellular biology · 2000Article
- Transient expression of DNA and RNA in parasitic helminths by using particle bombardment.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- SR proteins are required for nematode trans-splicing in vitro.RNA (New York, N.Y.) · 1999Article
- Alterations in the conserved SL1 trans-spliced leader of Caenorhabditis elegans demonstrate flexibility in length and sequence requirements in vivo.Molecular and cellular biology · 1999Article
- Structure-function analysis of the trypanosomatid spliced leader RNA.Nucleic acids research · 1998Article
- In vivo function of mutated spliced leader RNAs in Caenorhabditis elegans.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Operons and SL2 trans-splicing exist in nematodes outside the genus Caenorhabditis.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Spliced leader RNA of trypanosomes: in vivo mutational analysis reveals extensive and distinct requirements for trans splicing and cap4 formation.The EMBO journal · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The spliced leader RNAs of both trypanosomes and nematodes can form similar secondary structures where the trans-splice donor site is involved in intramolecular base pairing with the spliced leader sequence. It has been proposed that this base pairing could serve to activate autonomously the SL RNA splice donor site. Here, we have examined exon requirements for trans-splicing in a nematode cell free system. Complete disruption of secondary structure interactions at and around the trans-splice donor site did not affect the ability of the SL RNA to function in trans-splicing. In addition, the highly conserved 22 nt sequence could be productively replaced by artificial exons ranging in size from 2 to 246 nucleotides. These results reinforce the view that the 'intron' portion of the SL RNA functions as an independent Sm snRNP whose role is to deliver exon sequences to the trans-spliceosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.