ArticleThe EMBO journal1991
Newly synthesized synaptophysin is transported to synaptic-like microvesicles via constitutive secretory vesicles and the plasma membrane.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 182 citations in OpenAlex.
- Synaptobrevin-2 Containing Extracellular Vesicles Are Rapidly Incorporated Into Mammalian Neurons via a Dynamin-Dependent Pathway.Journal of neurochemistry · 2026Article
- Formation, Organization, and Maintenance of Synaptic Vesicle Pools: From Molecules to Structure and Dynamics.Advances in neurobiology · 2026Review
- ATG9A vesicles are a subtype of intracellular nanovesicle.Journal of cell science · 2025Article
- N-glycoproteomics of brain synapses and synaptic vesicles.Cell reports · 2023Article
- Article
- Interplay between phosphorylation and palmitoylation mediates plasma membrane targeting and sorting of GAP43.Molecular biology of the cell · 2014Article
- Synaptic vesicle recycling: steps and principles.The EMBO journal · 2014Review
- Rab33a mediates anterograde vesicular transport for membrane exocytosis and axon outgrowth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- The association of dynamin with synaptophysin regulates quantal size and duration of exocytotic events in chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- A tyrosine-based motif localizes a Drosophila vesicular transporter to synaptic vesicles in vivo.The Journal of biological chemistry · 2010Article
- Ectopic synaptic ribbons in dendrites of mouse retinal ON- and OFF-bipolar cells.Cell and tissue research · 2009Article
- Subcellular localization of the antidepressant-sensitive norepinephrine transporter.BMC neuroscience · 2009Article
- Synaptic vesicle protein trafficking at the glutamate synapse.Neuroscience · 2009Review
- Trafficking of vesicular neurotransmitter transporters.Traffic (Copenhagen, Denmark) · 2008Review
- Synaptotagmin IV is necessary for the maturation of secretory granules in PC12 cells.The Journal of cell biology · 2006Article
- Entry of newly synthesized GLUT4 into the insulin-responsive storage compartment is GGA dependent.The EMBO journal · 2004Article
- Synaptophysin I controls the targeting of VAMP2/synaptobrevin II to synaptic vesicles.Molecular biology of the cell · 2003Article
- Signals involved in targeting membrane proteins to synaptic vesicles.Cellular and molecular neurobiology · 2002Review
- A conserved mechanism of synaptogyrin localization.Molecular biology of the cell · 2001Article
- Neurosecretory granule formation in ligated axons: additional arguments for a local differentiation from a Golgi apparatus extension.The Histochemical journal · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biogenesis of synaptic-like microvesicles (SLMVs) in neuroendocrine cells was investigated by studying the traffic of newly synthesized synaptophysin to SLMVs in PC12 cells. Synaptophysin was found to be sulfated, which facilitated the determination of its exit route from the trans-Golgi network (TGN). Virtually all [35S]sulfate-labeled synaptophysin was found to leave the TGN in vesicles which were indistinguishable from constitutive secretory vesicles but distinct from immature secretory granules and SLMVs. [35S]sulfate-labeled synaptophysin was rapidly transported from the TGN to the cell surface, with a t1/2 of approximately 10 min in resting cells. After arrival at the cell surface, [35S]sulfate-labeled synaptophysin cycled for at least 1 h between the plasma membrane and an intracellular compartment likely to be the early endosome. Up to approximately 40% of the [35S]sulfate-labeled synaptophysin eventually (after 3 h and later) reached SLMVs, which could be distinguished from the other post-TGN compartments by their lower buoyant density in a sucrose gradient and their selective inclusion upon permeation chromatography using a controlled-pore glass column. Our results suggest that newly synthesized membrane proteins of SLMVs in neuroendocrine cells, and possibly of small synaptic vesicles in neurons, reach these organelles via the TGN----plasma membrane----early endosome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.