ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2009
Development and analysis of EST-SSRs for flax (Linum usitatissimum L.).
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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56 citing papers in PubMed, 165 citations in OpenAlex.
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- Simple Sequence Repeats (SSRs) and Telomeric Analysis in Somatic Organs of Reproductive and Non-Reproductive Castes of TermiteBiology · 2025Article
- Kallfu and Wenutram: two Chilean flaxseed varieties with contrasting mucilage production, composition, and structure.Frontiers in plant science · 2025Article
- Genetic Diversity ofPlants (Basel, Switzerland) · 2024Article
- History and prospects of flax genetic markers.Frontiers in plant science · 2024Review
- Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges.Molecular biology reports · 2023Article
- Review
- Diversity ofFrontiers in nutrition · 2023Review
- SSR markers development and their application in genetic diversity evaluation of garlic (Plant diversity · 2022Article
- Fine-mapping of a putative glutathione S-transferase (GST) gene responsible for yellow seed colour in flax (Linum usitatissimum).BMC research notes · 2022Article
- Integrated omics approaches for flax improvement under abiotic and biotic stress: Current status and future prospects.Frontiers in plant science · 2022Review
- Mapping Quantitative Trait Loci onto Chromosome-Scale Pseudomolecules in Flax.Methods and protocols · 2020Article
- Draft genome of multiple resistance donor plant Sinapis alba: An insight into SSRs, annotations and phylogenetics.PloS one · 2020Article
- Genome-wide regulatory gene-derived SSRs reveal genetic differentiation and population structure in fiber flax genotypes.Journal of applied genetics · 2019Article
- Genome-Wide Association Analysis of Mucilage and Hull Content in Flax (International journal of molecular sciences · 2018Article
- Development and Characterization of High-Throughput EST-Based SSR Markers forMolecules (Basel, Switzerland) · 2018Article
- Consensus genetic linkage map construction and QTL mapping for plant height-related traits in linseed flax (Linum usitatissimum L.).BMC plant biology · 2018Article
- Identification of Putative Precursor Genes for the Biosynthesis of Cannabinoid-Like Compound inFrontiers in plant science · 2018Article
- QTL mapping and molecular characterization of the classical D locus controlling seed and flower color in Linum usitatissimum (flax).Scientific reports · 2017Article
- Genetic diversity, population structure and association analysis in linseed (Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A set of 146,611 expressed sequence tags (ESTs) were generated from 10 flax cDNA libraries. After assembly, a total of 11,166 contigs and 11,896 singletons were mined for the presence of putative simple sequence repeats (SSRs) and yielded 806 (3.5%) non-redundant sequences which contained 851 putative SSRs. This is equivalent to one EST-SSR per 16.5 kb of sequence. Trinucleotide motifs were the most abundant (76.9%), followed by dinucleotides (13.9%). Tetra-, penta- and hexanucleotide motifs represented <10% of the SSRs identified. A total of 83 SSR motifs were identified. Motif (TTC/GAA)n was the most abundant (10.2%) followed by (CTT/AAG)n (8.7%), (TCT/AGA)n (8.6%), (CT/AG)n (6.7%) and (TC/GA)n (5.3%). A total of 662 primer pairs were designed, of which 610 primer pairs yielded amplicons in a set of 23 flax accessions. Polymorphism between the accessions was found for 248 primer pairs which detected a total of 275 EST-SSR loci. Two to seven alleles were detected per marker. The polymorphism information content value for these markers ranged from 0.08 to 0.82 and averaged 0.35. The 635 alleles detected by the 275 polymorphic EST-SSRs were used to study the genetic relationship of 23 flax accessions. Four major clusters and two singletons were observed. Sub-clusters within the main clusters correlated with the pedigree relationships amongst accessions. The EST-SSRs developed herein represent the first large-scale development of SSR markers in flax. They have potential to be used for the development of genetic and physical maps, quantitative trait loci mapping, genetic diversity studies, association mapping and fingerprinting cultivars for example.
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