ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2009
Housekeeping gene stability in pristane-induced arthritis and antigen-induced pulmonary inflammation of rats.
Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 11 citations in OpenAlex.
- Insensitive Effects of Inflammatory Cytokines on the Reference Genes of Synovial Fluid Resident-Mesenchymal Stem Cells Derived from Rheumatoid Arthritis Patients.International journal of molecular sciences · 2023Article
- Stability of housekeeping genes in inflamed joints of spontaneous and collagen-induced arthritis in DBA/1 mice.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2021Article
- Gapdh gene expression is modulated by inflammatory arthritis and is not suitable for qPCR normalization.Inflammation · 2014Article
- The effect of hypothermia on the expression of TIMP-3 after traumatic brain injury in rats.Journal of neurotrauma · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
OBJECTIVE AND
designAim of this study is to explore the expression stability of 12 commonly used housekeeping genes in inflammation condition. MATERIALS AND
methodsTwo rat models, pristane-induced arthritis (PIA) and antigen-induced pulmonary inflammation (AIPI) were established and reverse transcription-PCR was performed from rat spleens and inguinal lymph nodes.
resultsThe reference gene expression fluctuated in the immune-associated tissues and 18S rRNA expression from the various inflammatory conditions was the most stably in all examined gene expression. Besides, peptidylprolyl isomerase A and beta-glucuronidase in PIA spleens, beta-actin and GAPDH in PIA inguinal lymph nodes, and peptidylprolyl isomerase A and beta-actin in AIPI spleens, expressed more stably than others. These results were further confirmed by expression study of TNF-alpha in PIA rat inguinal lymph nodes.
conclusionWe suggest that the stable gene mentioned above or geometric mean of three best references in each experimental condition should be used as the reference and the variable ones should be avoided in similar study designs.
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