ArticleAmerican journal of physiology. Renal physiology2009
Podocyte-specific expression of organic cation transporter PMAT: implication in puromycin aminonucleoside nephrotoxicity.
Article in American journal of physiology. Renal physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Novel emerging cell and organoid systems for the study of drug metabolism and toxicity in humans.Drug metabolism and disposition: the biological fate of chemicals · 2025Review
- ATP/ADP biosensor organoids for drug nephrotoxicity assessment.Frontiers in cell and developmental biology · 2023Article
- The Role ofNon-coding RNA · 2022Article
- Membrane Carriers and Transporters in Kidney Physiology and Disease.Biomedicines · 2021Review
- Article
- mRNAs in urinary nano-extracellular vesicles as potential biomarkers for non-invasive kidney biopsy.Biomedical reports · 2021Article
- Substrates and Inhibitors of Organic Cation Transporters (OCTs) and Plasma Membrane Monoamine Transporter (PMAT) and Therapeutic Implications.Handbook of experimental pharmacology · 2021Article
- Advances in predictive in vitro models of drug-induced nephrotoxicity.Nature reviews. Nephrology · 2018Review
- The plasma membrane monoamine transporter (PMAT): Structure, function, and role in organic cation disposition.Clinical pharmacology and therapeutics · 2016Review
- Small molecule membrane transporters in the mammalian podocyte: a pathogenic and therapeutic target.International journal of molecular sciences · 2014Review
- Impaired monoamine and organic cation uptake in choroid plexus in mice with targeted disruption of the plasma membrane monoamine transporter (Slc29a4) gene.The Journal of biological chemistry · 2013Article
- Podocyte expression of membrane transporters involved in puromycin aminonucleoside-mediated injury.PloS one · 2013Article
- Residue Ile89 in human plasma membrane monoamine transporter influences its organic cation transport activity and sensitivity to inhibition by dilazep.Biochemical pharmacology · 2012Article
- Electrophysiological characterization of the polyspecific organic cation transporter plasma membrane monoamine transporter.Drug metabolism and disposition: the biological fate of chemicals · 2012Article
- Expression Profiling of Solute Carrier Gene Families at the Blood-CSF Barrier.Frontiers in pharmacology · 2012Article
- Molecular analysis and structure-activity relationship modeling of the substrate/inhibitor interaction site of plasma membrane monoamine transporter.The Journal of pharmacology and experimental therapeutics · 2011Article
- The contribution of low-affinity transport mechanisms to serotonin clearance in synaptosomes.Synapse (New York, N.Y.) · 2011Article
- Effect of phospholipidosis on the cellular pharmacokinetics of chloroquine.The Journal of pharmacology and experimental therapeutics · 2011Article
- Clinical pharmacokinetics of metformin.Clinical pharmacokinetics · 2011 · on this mapReview
- Tyrosine 112 is essential for organic cation transport by the plasma membrane monoamine transporter.Biochemistry · 2010Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Plasma membrane monoamine transporter (PMAT) is a novel polyspecific organic cation transporter that transports organic cations and the purine nucleoside, adenosine. PMAT is expressed in the kidney, but the specific localization and function of this transporter in renal cells are unclear. In this study, we developed a polyclonal antibody toward a 14-amino acid sequence in the last intracellular loop of PMAT and determined the precise cellular localization of PMAT in human and rat kidneys. Surprisingly, we found that the PMAT protein was predominantly expressed in the glomerulus with minimal expression in tubular cells. Within the glomerulus, dual-color immunofluorescence labeling showed that the PMAT protein was specifically localized to the visceral glomerular epithelial cells, i.e., podocytes. There was no significant PMAT immunoreactivity in mesangial or glomerular endothelial cells. We further showed that puromycin aminonucleoside (PAN), a classic podocyte toxin that induces massive proteinuria and severe glomerulopathy, is transported by PMAT. Expression of PMAT in Madin-Darby canine kidney cells significantly increased cell sensitivity to PAN. Decynium 22, a potent PMAT inhibitor, abolished PAN toxicity in PMAT-expressing cells. Together, our data suggest that PMAT is specifically expressed in podocytes and may play an important role in PAN-induced kidney injury.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.