ArticleNucleic acids research2009
A genotype-to-phenotype map of in vitro selected RNA-cleaving DNAzymes: implications for accessing the target phenotype.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Systematic minimization of RNA ligase ribozyme through large-scale design-synthesis-sequence cycles.Nucleic acids research · 2019Article
- Nucleobase modification by an RNA enzyme.Nucleic acids research · 2017Article
- DNA Catalysis: The Chemical Repertoire of DNAzymes.Molecules (Basel, Switzerland) · 2015Review
- High-throughput assay and engineering of self-cleaving ribozymes by sequencing.Nucleic acids research · 2015Article
- Comprehensive experimental fitness landscape and evolutionary network for small RNA.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A small ribozyme with dual-site kinase activity.Nucleic acids research · 2012Article
- In vitro selection of metal ion-selective DNAzymes.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Quasispecies-like behavior observed in catalytic RNA populations evolving in a test tube.BMC evolutionary biology · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
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Abstract
Herein, we describe a case study into the population dynamics of in vitro selection, using RNA-cleaving DNAzymes as a model system. We sought to understand how the composition of the population can change over time in response to different levels of selection pressure, and how well these changes are correlated with selection of the target phenotype. The model population is composed of 857 DNAzyme clones representing 215 discrete sequence classes, which had previously been identified from two parallel selection experiments, conducted under an increasingly stringent, or permissive and constant selection time pressure. In this report, we determined the principal phenotypic properties (i.e. k(obs), maximum cleavage yield and PCR efficiency) from a sample of 58 clones representing 46 different major and minor sequence classes from various rounds of each selection experiment. Interestingly, a positive correlation between the catalytic rate constant and the corresponding frequency and temporal position of a given DNAzyme was not consistently observed; however, the strength of the correlation was qualitatively higher under conditions of more stringent selection time pressure. These results suggest that the selective sampling paradigm on which in vitro selection is based, may underestimate the true functional capacity of any given random-sequence library.
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