ArticleJournal of the American Chemical Society2009
Laterally mobile, functionalized self-assembled monolayers at the fluorous-aqueous interface in a plug-based microfluidic system: characterization and testing with membrane protein crystallization.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Synthetic Biology-Empowered Hydrogels for Medical Diagnostics.Advances in biochemical engineering/biotechnology · 2021Article
- A microfluidic-based bubble generation platform enables analysis of physical property change in phospholipid surfactant layers by interfacial ozone reaction.Lab on a chip · 2012Article
- Control of initiation, rate, and routing of spontaneous capillary-driven flow of liquid droplets through microfluidic channels on SlipChip.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator.Journal of the American Chemical Society · 2011Article
- Challenges and opportunities for new protein crystallization strategies in structure-based drug design.Expert opinion on drug discovery · 2010Review
- Nanoliter multiplex PCR arrays on a SlipChip.Analytical chemistry · 2010Article
- A Stochastic Model for Nucleation Kinetics Determination in Droplet-Based Microfluidic Systems.Crystal growth & design · 2010Article
- SlipChip for immunoassays in nanoliter volumes.Analytical chemistry · 2010Article
- Evolution of catalysts directed by genetic algorithms in a plug-based microfluidic device tested with oxidation of methane by oxygen.Journal of the American Chemical Society · 2010Article
- Microfluidic stochastic confinement enhances analysis of rare cells by isolating cells and creating high density environments for control of diffusible signals.Chemical Society reviews · 2010Review
- User-loaded SlipChip for equipment-free multiplexed nanoliter-scale experiments.Journal of the American Chemical Society · 2010Article
- SlipChip.Lab on a chip · 2009Article
- Plug-based microfluidics with defined surface chemistry to miniaturize and control aggregation of amyloidogenic peptides.Angewandte Chemie (International ed. in English) · 2009Article
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Authors and funding
5 authors.
Funding
Abstract
This paper describes a method to generate functionalizable, mobile self-assembled monolayers (SAMs) in plug-based microfluidics. Control of interfaces is advancing studies of biological interfaces, heterogeneous reactions, and nanotechnology. SAMs have been useful for such studies, but they are not laterally mobile. Lipid-based methods, though mobile, are not easily amenable to setting up the hundreds of experiments necessary for crystallization screening. Here we demonstrate a method, complementary to current SAM and lipid methods, for rapidly generating mobile, functionalized SAMs. This method relies on plugs, droplets surrounded by a fluorous carrier fluid, to rapidly explore chemical space. Specifically, we implemented his-tag binding chemistry to design a new fluorinated amphiphile, RfNTA, using an improved one-step synthesis of RfOEG under Mitsunobu conditions. RfNTA introduces specific binding of protein at the fluorous-aqueous interface, which concentrates and orients proteins at the interface, even in the presence of other surfactants. We then applied this approach to the crystallization of a his-tagged membrane protein, Reaction Center from Rhodobacter sphaeroides, performed 2400 crystallization trials, and showed that this approach can increase the range of crystal-producing conditions, the success rate at a given condition, the rate of nucleation, and the quality of the crystal formed.
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