ArticleJournal of Zhejiang University. Science. B2009
A system for screening agonists targeting beta2-adrenoceptor from Chinese medicinal herbs.
Article in Journal of Zhejiang University. Science. B, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 10 citations in OpenAlex.
- UPLC-MS/MS Method for the Simultaneous Quantification of Eight Compounds in Rat Plasma and Its Application to a Pharmacokinetic Study after Oral Administration of Veratrum (BioMed research international · 2020Article
- Veratri Nigri Rhizoma et Radix (Veratrum nigrum L.) and Its Constituent Jervine Prevent Adipogenesis via Activation of the LKB1-AMPKα-ACC Axis In Vivo and In Vitro.Evidence-based complementary and alternative medicine : eCAM · 2016Article
- Article
- Interaction of Veratrum nigrum with Panax ginseng against Obesity: A Sang-ban Relationship.Evidence-based complementary and alternative medicine : eCAM · 2013Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to develop a model for screening the agonists of human beta(2)-adrenoceptor from Chinese medicinal herbs extracts, we used a cell-based functional assay based on a common G protein-coupled receptor (GPCR) regulation mechanism and destabilized enhanced green fluorescent protein (d(2)EGFP) reporter gene technique. The positive cell clone was confirmed by real-time polymerase chain reaction (PCR) and imaging analysis. To assess the value of this model, we screened over 2000 high performance liquid chromatography (HPLC)-fractionated samples from the ethanol extracts of Chinese medicinal herbs. Six fractions (isolated from Panax japonicus, Veratrum nigrum, Phellodendron amurense, Fructus Aurantii Immaturus, Chaenomeles speciosa, and Dictamnus dasycarpus) showed significant effects on active reporter gene expression, three of which (isolated from Phellodendron amurense, Fructus Aurantii Immaturus, and Chaenomeles speciosa) were selected for further concentration response analysis and the half maximal effective concentration (EC(1/2 max)) values were 4.2, 2.7, and 4.8 microg/ml, respectively. Therefore, this reporter gene assay was suitable for screening beta(2)-adrenoceptor agonists. The results suggest that the six herbal extracts are the possible agonists of beta(2)-adrenoceptor.
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