ArticleEuropean journal of nuclear medicine and molecular imaging2009
Characterization of choline uptake in prostate cancer cells following bicalutamide and docetaxel treatment.
Article in European journal of nuclear medicine and molecular imaging, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- [International journal of molecular sciences · 2021Article
- Article
- Functional Expression of Choline Transporter-Like Protein 1 in LNCaP Prostate Cancer Cells: A Novel Molecular Target.Biomolecules & therapeutics · 2020Article
- Preclinical Evaluation of Radiolabeled Peptides for PET Imaging of Glioblastoma Multiforme.Molecules (Basel, Switzerland) · 2019Article
- Detection Rate ofJournal of nuclear medicine : official publication, Society of Nuclear Medicine · 2019Article
- Dietary choline and betaine intakes and risk of total and lethal prostate cancer in the Atherosclerosis Risk in Communities (ARIC) Study.Cancer causes & control : CCC · 2019Article
- PET and PET/CT with radiolabeled choline in prostate cancer: a critical reappraisal of 20 years of clinical studies.European journal of nuclear medicine and molecular imaging · 2017Review
- Interpretation of 11C-choline PET/CT for the diagnosis of local relapse in radically treated prostate cancer.European journal of hybrid imaging · 2017Article
- [18F]fluoromethylcholine as a chemotherapy response read-out in prostate cancer cells.Molecular imaging and biology · 2015Article
- Comparison of [¹¹C]choline ([¹¹C]CHO) and S(+)-β-methyl-[¹¹C]choline ([¹¹C]SMC) as imaging probes for prostate cancer in a PC-3 prostate cancer xenograft model.Molecular imaging and biology · 2015Article
- Furosemide diminishes ¹⁸F-fluoroethylcholine uptake in prostate cancer in vivo.European journal of nuclear medicine and molecular imaging · 2014Article
- Recent Advances in Metabolic Profiling And Imaging of Prostate Cancer.Current Metabolomics · 2014Article
- Role of focal salvage ablative therapy in localised radiorecurrent prostate cancer.World journal of urology · 2013Review
- Targeting free prostate-specific antigen for in vivo imaging of prostate cancer using a monoclonal antibody specific for unique epitopes accessible on free prostate-specific antigen alone.Cancer biotherapy & radiopharmaceuticals · 2012Article
- Relationship between PSA kinetics and [18F]fluorocholine PET/CT detection rates of recurrence in patients with prostate cancer after total prostatectomy.European journal of nuclear medicine and molecular imaging · 2012Article
- Do androgens control the uptake of 18F-FDG, 11C-choline and 11C-acetate in human prostate cancer cell lines?European journal of nuclear medicine and molecular imaging · 2011Article
- Human prostate cell lines from normal and tumourigenic epithelia differ in the pattern and control of choline lipid headgroups released into the medium on stimulation of protein kinase C.British journal of cancer · 2011Article
- [11C]Choline as pharmacodynamic marker for therapy response assessment in a prostate cancer xenograft model.European journal of nuclear medicine and molecular imaging · 2010Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeCholine derivatives labelled with positron emitters are successfully used for PET imaging of prostate cancer patients. Since little is known about uptake mechanisms, the aim of this study was to characterize choline uptake in prostate cancer cells, also following anti-androgen treatment or chemotherapy.
methodsCholine uptake in prostate cancer cells (LNCaP, PC-3) and Michaelis-Menten kinetics were analysed using different concentrations of (3)H-choline via liquid scintillation counting. Inhibition of (3)H-choline uptake was assayed in the presence of hemicholinium-3 (HC-3), unlabelled choline, guanidine and tetraethylammonium (TEA), an inhibitor of the organic cation transporter (OCT). Changes in choline uptake triggered by bicalutamide and docetaxel were evaluated and choline transporters were detected via Western blotting.
resultsMichaelis-Menten kinetics yielded a saturable transport with K(m) values of 6.9 and 7.0 micromol/l choline for LNCaP and PC-3 cells, respectively. Treatment of cells with bicalutamide and docetaxel caused an increase in total choline uptake but had no significant effect on K(m) values. Uptake of (3)H-choline was NaCl dependent and 4.5-fold higher in LNCaP cells than in PC-3 cells. (3)H-Choline uptake was reduced by 92-96% using HC-3 and unlabelled choline, by 63-69% using guanidine and by 20% using TEA. The high-affinity choline transporter was detected via Western blotting.
conclusionCholine uptake in prostate cancer cells is accomplished both by a transporter-mediated and a diffusion-like component. Results of inhibition experiments suggest that uptake is mediated by a selective choline transporter rather than by the OCT. Bicalutamide- and docetaxel-induced changes in total choline uptake could affect PET tumour imaging.
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