ArticleMolecular therapy : the journal of the American Society of Gene Therapy2009
Enhancement of myogenic and muscle repair capacities of human adipose-derived stem cells with forced expression of MyoD.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 121 citations in OpenAlex.
- A new method for treating fecal incontinence by implanting stem cells derived from human adipose tissue: preliminary findings of a randomized double-blind clinical trial.Stem cell research & therapy · 2017Trial
- Restoration of myogenesis in ALS-myocytes through miR-26a-5p-mediated Smad4 inhibition and its impact on motor neuron development.Molecular therapy. Nucleic acids · 2025Article
- Transcriptomic analysis of bone transport reveals different functions between both ends.Frontiers in physiology · 2025Article
- Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering.International journal of molecular sciences · 2024Review
- Advances in Genetic Reprogramming: Prospects from Developmental Biology to Regenerative Medicine.Current medicinal chemistry · 2024Review
- Stromal vascular fraction in the treatment of myositis.Cell death discovery · 2023Review
- Bioprocessing Considerations towards the Manufacturing of Therapeutic Skeletal and Smooth Muscle Cells.Bioengineering (Basel, Switzerland) · 2023Review
- The one-step fabrication of porous hASC-laden GelMa constructs using a handheld printing system.NPJ Regenerative medicine · 2023Article
- Refining theAnimals : an open access journal from MDPI · 2022Article
- Recent advances toward understanding the role of transplanted stem cells in tissue-engineered regeneration of musculoskeletal tissues.F1000Research · 2020Review
- Bio-instructive materials for musculoskeletal regeneration.Acta biomaterialia · 2019Review
- Skeletal Muscle Regenerative Engineering.Regenerative engineering and translational medicine · 2019Article
- Current Strategies to Enhance Adipose Stem Cell Function: An Update.International journal of molecular sciences · 2019Review
- Enhancing the Therapeutic Potential of Mesenchymal Stem Cells with the CRISPR-Cas System.Stem cell reviews and reports · 2019Review
- Human induced pluripotent stem cell models for the study and treatment of Duchenne and Becker muscular dystrophies.Therapeutic advances in neurological disorders · 2019Review
- Skeletal Muscle Regenerative Potential of Human MuStem Cells following Transplantation into Injured Mice Muscle.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Myogenic regulatory factors: The orchestrators of myogenesis after 30 years of discovery.Experimental biology and medicine (Maywood, N.J.) · 2018Review
- An Engineered Optogenetic Switch for Spatiotemporal Control of Gene Expression, Cell Differentiation, and Tissue Morphogenesis.ACS synthetic biology · 2017Article
- Tat-MyoD fused proteins, together with C2c12 conditioned medium, are able to induce equine adult mesenchimal stem cells towards the myogenic fate.Veterinary research communications · 2017Article
- Adipose-derived stem cells enhance myogenic differentiation in the mdx mouse model of muscular dystrophyNeural regeneration research · 2016Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Muscle disorders such as Duchenne muscular dystrophy (DMD) still need effective treatments, and mesenchymal stem cells (MSCs) may constitute an attractive cell therapy alternative because they are multipotent and accessible in adult tissues. We have previously shown that human multipotent adipose-derived stem (hMADS) cells were able to restore dystrophin expression in the mdx mouse. The goal of this work was to improve the myogenic potential of hMADS cells and assess the impact on muscle repair. Forced expression of MyoD in vitro strongly induced myogenic differentiation while the adipogenic differentiation was inhibited. Moreover, MyoD-expressing hMADS cells had the capacity to fuse with DMD myoblasts and to restore dystrophin expression. Importantly, transplantation of these modified hMADS cells into injured muscles of immunodepressed Rag2(-/-)gammaC(-/-) mice resulted in a substantial increase in the number of hMADS cell-derived fibers. Our approach combined the easy access of MSCs from adipose tissue, the highly efficient lentiviral transduction of these cells, and the specific improvement of myogenic differentiation through the forced expression of MyoD. Altogether our results highlight the capacity of modified hMADS cells to contribute to muscle repair and their potential to deliver a repairing gene to dystrophic muscles.
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