ArticleJournal of the American Chemical Society2009
Rapid and simple ribozymic aminoacylation using three conserved nucleotides.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 71 citations in OpenAlex.
- Yield and fidelity of early steps of ribosome-free translation depend on the RNA anchoring.Nucleic acids research · 2026Article
- Complex and Messy Prebiotic Chemistry: Obstacles and Opportunities for an RNA World.Life (Basel, Switzerland) · 2026Review
- Translation-Promoting Effects of RNA Template Overhangs in the Absence of Ribosomes.Angewandte Chemie (International ed. in English) · 2026Article
- Structural Enzymology, Phylogenetics, Differentiation, and Symbolic Reflexivity at the Dawn of Biology.Genome biology and evolution · 2025Article
- Assembly of catalytic complexes from randomized oligonucleotides.Science advances · 2025Article
- An anticodon-sensing T-boxzyme generates the elongator nonproteinogenic aminoacyl-tRNA in situ of a custom-made translation system for incorporation.Nucleic acids research · 2024Article
- Discovering pathways through ribozyme fitness landscapes using information theoretic quantification of epistasis.RNA (New York, N.Y.) · 2023Article
- Triplet-Encoded Prebiotic RNA Aminoacylation.Journal of the American Chemical Society · 2023Article
- Efficient Oligomerization of Aromatic Amino Acids Induced by Gaps in Four-Helix Bundles of DNA or RNA.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Nonenzymatic assembly of active chimeric ribozymes from aminoacylated RNA oligonucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Fitting the standard genetic code into its triplet table.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Possible Ancestral Functions of the Genetic and RNA Operational Precodes and the Origin of the Genetic System.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2021Article
- Evolution of the Standard Genetic Code.Journal of molecular evolution · 2021Article
- A combinatorial method to isolate short ribozymes from complex ribozyme libraries.Nucleic acids research · 2020Article
- The 3-Minihelix tRNA Evolution Theorem.Journal of molecular evolution · 2020Article
- Evolution of Life on Earth: tRNA, Aminoacyl-tRNA Synthetases and the Genetic Code.Life (Basel, Switzerland) · 2020Review
- Dynamics and stability in prebiotic information integration: an RNA World model from first principles.Scientific reports · 2020Article
- A tRNA- and Anticodon-Centric View of the Evolution of Aminoacyl-tRNA Synthetases, tRNAomes, and the Genetic Code.Life (Basel, Switzerland) · 2019Review
- Mapping a Systematic Ribozyme Fitness Landscape Reveals a Frustrated Evolutionary Network for Self-Aminoacylating RNA.Journal of the American Chemical Society · 2019Article
- Type-II tRNAs and Evolution of Translation Systems and the Genetic Code.International journal of molecular sciences · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Selection-amplification finds new RNA enzymes (ribozymes) among randomized RNAs with flanking unvaried sequences (primer complements). Precise removal of 3'-primer before reaction selected aminoacylation from PheAMP in three cycles, yielding active RNAs (k(cat) = 12-20 min(-1)) using only three conserved nucleotides, acting independently of divalent ions. This unusually simple RNA active site encouraged study of the reaction via molecular mechanics-based free energy minimization. On this basis, we suggest a chemical path for RNA-catalyzed transaminoacylation. Site modeling also predicted new features, L-stereoselectivity, 2'-regioselectivity, independence of amino acid side chain, and phosphorylated activating group, that were subsequently verified. The same selection also showed that RNA aminoacylation from adenylate is simpler than from CoA thioester, potentially rationalizing translational activation by adenylates. The simplicity of this active site suggests a general route to small ribozymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.