ArticleJournal of industrial microbiology & biotechnology2009
Medium optimization for keratinase production in hair substrate by a new Bacillus subtilis KD-N2 using response surface methodology.
Article in Journal of industrial microbiology & biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 106 citations in OpenAlex.
- Biodegradation of hair waste for keratinase production using Bacillus altitudinis VK-1120 and its application in enzymatic dehairing.Biodegradation · 2026Article
- Purification, characterization, and dehairing properties of alkaline and thermo-stable keratinase by Penicillium citrinum AUMC 14742.Scientific reports · 2026Article
- In vitro evaluation of Bacillus subtilis isolated from Apis mellifera for potential use as a probiotic feed additive in honeybees.Scientific reports · 2025Article
- Enhancement of Menaquinone-7 production in Bacillus subtilis by optimizing the medium components through response surface methodology.Bioresources and bioprocessing · 2025Article
- Biodegradation of Keratin Waste by Bacillus velezensis HFS_F2 through Optimized Keratinase Production Medium.Current microbiology · 2024Article
- Production of surfactant-stable keratinase from Bacillus cereus YQ15 and its application as detergent additive.BMC biotechnology · 2022Article
- Optimization of Conditions for Feather Waste Biodegradation by GeophilicInternational journal of environmental research and public health · 2022Article
- A novel feather-degrading bacterial isolate Geobacillus thermodenitrificans PS41 isolated from poultry farm soil.Archives of microbiology · 2022Article
- Enhanced keratinase production by3 Biotech · 2022Article
- Bacillus sp. CSK2 produced thermostable alkaline keratinase using agro-wastes: keratinolytic enzyme characterization.BMC biotechnology · 2020Article
- Biochemical and Molecular Characterization of a Thermostable Alkaline Metallo-Keratinase fromMicroorganisms · 2020Article
- Azo dying of α-keratin material improves microbial keratinase screening and standardization.Microbial biotechnology · 2020Article
- Recombinant expression and biochemical characterization of a novel keratinase BsKER71 from feather degrading bacterium Bacillus subtilis S1-4.AMB Express · 2020Article
- Biotransformation of keratin waste to amino acids and active peptides based on cell-free catalysis.Biotechnology for biofuels · 2020Article
- Removal of chromium from wastewater by swine hair residues applied as a putative biofilter.Environmental science and pollution research international · 2019Article
- Biodegradation of feather waste by keratinase produced from newly isolatedJournal, genetic engineering & biotechnology · 2018Article
- New keratinolytic bacteria in valorization of chicken feather waste.AMB Express · 2018Article
- Decolorization of textile dye RB19 using volcanic rock matrix immobilized Bacillus thuringiensis cells with surface displayed laccase.World journal of microbiology & biotechnology · 2017Article
- Keratin Durability Has Implications for the Fossil Record: Results from a 10 Year Feather Degradation Experiment.PloS one · 2016Article
- Biodegradation of hard keratins by two bacillus strains.Jundishapur journal of microbiology · 2014Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culture medium for keratinase production from hair substrate by a new Bacillus subtilis strain, KD-N2, was optimized. Effects of culture conditions on keratinase production were tested, and optimal results were obtained with 10% inocula (v/v), 16 g/L hair substrate, an initial pH value of 6.5 and a culture volume of 20 mL. Several carbon sources (sucrose, cornflour) and nitrogen sources (yeast extract, tryptone and peptone) had positive effects on keratinase production, with sucrose giving optimal results. To improve keratinase yield, statistically based experimental designs were applied to optimize the culture medium. Fractional factorial design (FFD) experiments showed that MgSO4 and K2HPO4 were the most significant factors affecting keratinase production. Further central composite design (CCD) experiments indicated that the optimal MgSO4 and K2HPO4 concentrations were 0.91 and 2.38 g/L, respectively. Using an optimized fermentation medium (g/L: NaCl 1.0, CaCl2 0.05, KH2PO4 0.7, sucrose 3, MgSO4 0.91, K2HPO4 2.38), keratinase activity increased to 125 U/mL, an approximate 1.7-fold increase over the previous activity (75 U/mL). Human hair was degraded during the submerged cultivation.
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