ArticleNature nanotechnology2009
A highly sensitive and selective diagnostic assay based on virus nanoparticles.
Article in Nature nanotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 160 citations in OpenAlex.
- An RNase domain-dependent microRNA detection with DNA-spiked nanocage for accurate cancer diagnosis.Nature communications · 2026Article
- Review
- Review
- Assembly and Characterization of HBc Derived Virus-like Particles with Magnetic Core.Nanomaterials (Basel, Switzerland) · 2019Article
- A Metal Chelator as a Plasmonic Signal-Generation Superregulator for Ultrasensitive Colorimetric Bioassays of Disease Biomarkers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Nanoparticles for Signaling in Biodiagnosis and Treatment of Infectious Diseases.International journal of molecular sciences · 2018Review
- Human Albumin Fragments Nanoparticles as PTX Carrier for Improved Anti-cancer Efficacy.Frontiers in pharmacology · 2018Article
- Penicillin Detection byNanotheranostics · 2018Article
- Targeted Intracellular Delivery of Antibodies: The State of the Art.Frontiers in pharmacology · 2018Review
- Genetic Assembly of Double-Layered Fluorescent Protein Nanoparticles for Cancer Targeting and Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Article
- Dual Electrophoresis Detection System for Rapid and Sensitive Immunoassays with Nanoparticle Signal Amplification.Scientific reports · 2017Article
- Hydrophilic Polyelectrolyte Multilayers Improve the ELISA System: Antibody Enrichment and Blocking Free.Polymers · 2017Article
- Graphene Nanogrids FET Immunosensor: Signal to Noise Ratio Enhancement.Sensors (Basel, Switzerland) · 2016Article
- Protein Nanoparticles as Multifunctional Biocatalysts and Health Assessment Sensors.Current opinion in chemical engineering · 2016Article
- Intracellular delivery of antibodies by chimeric Sesbania mosaic virus (SeMV) virus like particles.Scientific reports · 2016Article
- Ultrasensitive microchip based on smart microgel for real-time online detection of trace threat analytes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A Wash-Free Homogeneous Colorimetric Immunoassay Method.Theranostics · 2016Article
- Aptamer-Phage Reporters for Ultrasensitive Lateral Flow Assays.Analytical chemistry · 2015Article
- Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection.Chemical Society reviews · 2015Review
- Virus outbreaks in chemical and biological sensors.Sensors (Basel, Switzerland) · 2014Review
Corrections and comments
- Commented on by
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early detection of the protein marker troponin I in patients with a higher risk of acute myocardial infarction can reduce the risk of death from heart attacks. Most troponin assays are currently based on the conventional enzyme linked immunosorbent assay and have detection limits in the nano- and picomolar range. Here, we show that by combining viral nanoparticles, which are engineered to have dual affinity for troponin antibodies and nickel, with three-dimensional nanostructures including nickel nanohairs, we can detect troponin levels in human serum samples that are six to seven orders of magnitude lower than those detectable using conventional enzyme linked immunosorbent assays. The viral nanoparticle helps to orient the antibodies for maximum capture of the troponin markers. High densities of antibodies on the surfaces of the nanoparticles and nanohairs lead to greater binding of the troponin markers, which significantly enhances detection sensitivities. The nickel nanohairs are re-useable and can reproducibly differentiate healthy serum from unhealthy ones. We expect other viral nanoparticles to form similar highly sensitive diagnostic assays for a variety of other protein markers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.