ArticleBiophysical journal2009
Sequence of physical changes to the cell membrane during glucocorticoid-induced apoptosis in S49 lymphoma cells.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Determination of Half-Maximal Inhibitory Concentration (ICAnalytical chemistry · 2025Article
- Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging.Scientific reports · 2024Article
- Revisiting the reaction pathways for phospholipid hydrolysis catalyzed by phospholipase A2 with QM/MM methods.Chemical science · 2024Article
- Apoptosis and eryptosis: similarities and differences.Apoptosis : an international journal on programmed cell death · 2024Review
- Corosolic Acid Induced Apoptosis via Upregulation of Bax/Bcl-2 Ratio and Caspase-3 Activation in Cholangiocarcinoma Cells.ACS omega · 2024Article
- Influenza A Virus Infection Alters Lipid Packing and Surface Electrostatic Potential of the Host Plasma Membrane.Viruses · 2023Article
- Characterization of the Peri-Membrane Fluorescence Phenomenon Allowing the Detection of Urothelial Tumor Cells in Urine.Cancers · 2022Article
- Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes.International journal of molecular sciences · 2022Article
- Mechanical Regulation of Apoptosis in the Cardiovascular System.Annals of biomedical engineering · 2021Review
- Quantifications of CSF Apoptotic Bodies Do Not Provide Clinical Value in Multiple Sclerosis.Frontiers in neurology · 2019Article
- Carboxybetaine-modified succinylated chitosan-based beads encourage pancreatic β-cells (Min-6) to form islet-like spheroids under in vitro conditions.Journal of materials science. Materials in medicine · 2017Article
- Analysis of Residual DSBs in Ataxia-Telangiectasia Lymphoblast Cells Initiating Apoptosis.BioMed research international · 2016Article
- Regulation of spontaneous eosinophil apoptosis-a neglected area of importance.Journal of cell death · 2014Review
- Membrane properties involved in calcium-stimulated microparticle release from the plasma membranes of S49 lymphoma cells.TheScientificWorldJournal · 2014Article
- Molecular details of membrane fluidity changes during apoptosis and relationship to phospholipase A(2) activity.Biochimica et biophysica acta · 2013Article
- Synergistic effects of secretory phospholipase A2 from the venom of Agkistrodon piscivorus piscivorus with cancer chemotherapeutic agents.BioMed research international · 2013Article
- Phosphatidylserine reversibly binds Cu2+ with extremely high affinity.Journal of the American Chemical Society · 2012Article
- Investigation into the role of phosphatidylserine in modifying the susceptibility of human lymphocytes to secretory phospholipase A(2) using cells deficient in the expression of scramblase.Biochimica et biophysica acta · 2012Article
- Relationship between membrane permeability and specificity of human secretory phospholipase A(2) isoforms during cell death.Biochimica et biophysica acta · 2011Article
- Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
During apoptosis, physical changes in the plasma membrane prepare the cell for clearance by phagocytes and hydrolysis by secretory phospholipase A(2) (sPLA(2)). The relationships among these changes have not been adequately established, especially for hormone-stimulated apoptosis. This study addresses these issues for glucocorticoid-induced apoptosis in S49 lymphoma cells. Flow cytometry, microscopy, and fluorescence spectroscopy were used to assess merocyanine 540 emission, laurdan generalized polarization, phosphatidylserine exposure, caspase activation, and membrane permeability to propidium iodide in the absence and presence of sPLA(2). The earliest event observed was activation of cellular caspases. Results with membrane probes suggest that interlipid spacing also increases early during apoptosis and precedes transbilayer migration of phosphatidylserine, DNA fragmentation, and a general increase in lipid order associated with blebbing and dissolution of the cells. The activity of sPLA(2) appeared to be linked more to lipid spacing than to loss of membrane asymmetry. The early nature of some of these events and their ability to promote activity of a proinflammatory enzyme suggests the possibility of an inflammatory response during T-lymphocyte apoptosis.
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