ArticlePloS one2009
The early apoptotic DNA fragmentation targets a small number of specific open chromatin regions.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Analysis of the role of acetylation inFrontiers in microbiology · 2024Article
- The "Genomic Code": DNA Pervasively Moulds Chromatin Structures Leaving no Room for "Junk".Life (Basel, Switzerland) · 2021Review
- Electronic DNA Analysis of CSF Cell-free Tumor DNA to Quantify Multi-gene Molecular Response in Pediatric High-grade Glioma.Clinical cancer research : an official journal of the American Association for Cancer Research · 2020Article
- Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment.Nucleic acids research · 2019Article
- Characterization of the microDNA through the response to chemotherapeutics in lymphoblastoid cell lines.PloS one · 2017Article
- Next-generation sequencing of apoptotic DNA breakpoints reveals association with actively transcribed genes and gene translocations.PloS one · 2011Article
- Isochores and the regulation of gene expression in the human genome.Genome biology and evolution · 2011Article
- Article
- The evolution of isochore patterns in vertebrate genomes.BMC genomics · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report here that early apoptotic DNA fragmentation, as obtained by using an entirely new approach, is the result of an attack at a small number of specific open chromatin regions of interphase nuclei. This was demonstrated as follows: (i) chicken liver was excised and kept in sterile tubes for 1 to 3 hours at 37 degrees C; (ii) this induced apoptosis (possibly because of oxygen deprivation), as shown by the electrophoretic nucleosomal ladder produced by DNA preparations; (iii) low molecular-weight DNA fragments (approximately 200 bp) were cloned, sequenced, and shown to derive predominantly from genes and surrounding 100 kb regions; (iv) a few hundred cuts were produced, very often involving the same chromosomal sites; (v) at comparable DNA degradation levels, micrococcal nuclease (MNase) also showed a general preference for genes and surrounding regions, but MNase cuts were located at sites that were quite distinct from, and less specific than, those cut by apoptosis. In conclusion, the approach presented here, which is the mildest and least intrusive approach, identifies a preferred accessibility landscape in interphase chromatin.
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