ArticlePlant physiology2009
Arabidopsis actin-depolymerizing factor AtADF4 mediates defense signal transduction triggered by the Pseudomonas syringae effector AvrPphB.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
91 citing papers in PubMed, 164 citations in OpenAlex.
- Review
- The conserved nematode pheromone ascr#18 primes plant immunity.Communications biology · 2026Article
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- Multi-omics insights into the molecular basis of powdery mildew resistance and root metabolic variation in Astragalus membranaceus var. mongholicus.Scientific reports · 2025Article
- Intracellular MIZU-KUSSEI1 movement and hydrotropism in Arabidopsis require F-actin organization.Plant physiology · 2025Article
- Calcium-dependent protein kinases in plant immunity: from calcium signaling to network integration.Frontiers in plant science · 2025Review
- MYB52 negatively regulates ADF9-meditated actin filament bundling in Arabidopsis pavement cell morphogenesis.Journal of integrative plant biology · 2024Article
- Identification of candidate genes for Fusarium head blight resistance from QTLs using RIL population in wheat.Plant molecular biology · 2024Article
- Actin-depolymerizing factors 8 and 11 promote root hair elongation at high pH.Plant communications · 2024Article
- Genome-wide identification of actin-depolymerizing factor family genes in melon (Frontiers in plant science · 2024Article
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- Actin-Depolymerizing Factor Gene Family Analysis Revealed ThatPlants (Basel, Switzerland) · 2023Article
- Prediction of Effector Proteins from Trichoderma longibrachiatum Through Transcriptome Sequencing.Current microbiology · 2023Article
- VILLIN2 regulates cotton defense against Verticillium dahliae by modulating actin cytoskeleton remodeling.Plant physiology · 2023Article
- Research progress on the roles of actin-depolymerizing factor in plant stress responses.Frontiers in plant science · 2023Review
- The Cytoskeleton in Plant Immunity: Dynamics, Regulation, and Function.International journal of molecular sciences · 2022Review
- Article
- Article
- Lipid Signaling Requires ROS Production to Elicit Actin Cytoskeleton Remodeling during Plant Innate Immunity.International journal of molecular sciences · 2022Article
- GhADF6-mediated actin reorganization is associated with defence against Verticillium dahliae infection in cotton.Molecular plant pathology · 2021Article
31 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The actin cytoskeleton has been implicated in plant defenses against pathogenic fungi and oomycetes with limited, indirect evidence. To date, there are no reports linking actin with resistance against phytopathogenic bacteria. The dynamic behavior of actin filaments is regulated by a diverse array of actin-binding proteins, among which is the Actin-Depolymerizing Factor (ADF) family of proteins. Here, we demonstrate that actin dynamics play a role in the activation of gene-for-gene resistance in Arabidopsis (Arabidopsis thaliana) following inoculation with the phytopathogenic bacterium Pseudomonas syringae pv tomato. Using a reverse genetics approach, we explored the roles of Arabidopsis ADFs in plant defenses. AtADF4 was identified as being specifically required for resistance triggered by the effector AvrPphB but not AvrRpt2 or AvrB. Recombinant AtADF4 bound to monomeric actin (G-actin) with a marked preference for the ADP-loaded form and inhibited the rate of nucleotide exchange on G-actin, indicating that AtADF4 is a bona fide actin-depolymerizing factor. Exogenous application of the actin-disrupting agent cytochalasin D partially rescued the Atadf4 mutant in the AvrPphB-mediated hypersensitive response, demonstrating that AtADF4 mediates defense signaling through modification of the actin cytoskeleton. Unlike the mechanism by which the actin cytoskeleton confers resistance against fungi and oomycetes, AtADF4 is not involved in resistance against pathogen entry. Collectively, this study identifies AtADF4 as a novel component of the plant defense signaling pathway and provides strong evidence for actin dynamics as a primary component that orchestrates plant defenses against P. syringae.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.