ArticleAmerican journal of physiology. Lung cellular and molecular physiology2009
The contribution of Ca2+ signaling and Ca2+ sensitivity to the regulation of airway smooth muscle contraction is different in rats and mice.
Article in American journal of physiology. Lung cellular and molecular physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 44 citations in OpenAlex.
- Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery.Respiratory research · 2024Review
- Inherent differences of small airway contraction and CaFrontiers in cell and developmental biology · 2023Article
- Perspectives on precision cut lung slices-powerful tools for investigation of mechanisms and therapeutic targets in lung diseases.Frontiers in pharmacology · 2023Review
- Airway smooth muscle function in asthma.Frontiers in physiology · 2022Review
- Stiffening of the extracellular matrix is a sufficient condition for airway hyperreactivity.Journal of applied physiology (Bethesda, Md. : 1985) · 2021Article
- Role of Airway Smooth Muscle in Inflammation Related to Asthma and COPD.Advances in experimental medicine and biology · 2021Article
- The role of diacylglycerol kinases in allergic airway disease.Current opinion in pharmacology · 2020Review
- Involvement of Allosteric Effect and KInternational journal of molecular sciences · 2018Article
- Purinergic receptor stimulation induces calcium oscillations and smooth muscle contraction in small pulmonary veins.The Journal of physiology · 2018Article
- Orosomucoid-like 3 (ORMDL3) upregulates airway smooth muscle proliferation, contraction, and CaThe Journal of allergy and clinical immunology · 2018Article
- Store-operated calcium entry is required for sustained contraction and CaThe Journal of physiology · 2017Article
- Involvement of CaInternational journal of molecular sciences · 2016Article
- Ryanodine receptor sensitization results in abnormal calcium signaling in airway smooth muscle cells.American journal of respiratory cell and molecular biology · 2015Article
- Relaxant action of plumula nelumbinis extract on mouse airway smooth muscle.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Differences in Akt signaling and metabolism gene expression in the right heart, intraventricular septum and left heart of rodents.International journal of clinical and experimental pathology · 2015Article
- MATHEMATICAL MODELING OF VENTILATION DEFECTS IN ASTHMA.Drug discovery today. Disease models · 2015Article
- Bitter tasting compounds dilate airways by inhibiting airway smooth muscle calcium oscillations and calcium sensitivity.British journal of pharmacology · 2014Article
- Abnormal tracheal smooth muscle function in the CF mouse.Physiological reports · 2013Article
- Adenosine A(1) and prostaglandin E receptor 3 receptors mediate global airway contraction after local epithelial injury.American journal of respiratory cell and molecular biology · 2013Article
- Ca2+ oscillations, Ca2+ sensitization, and contraction activated by protein kinase C in small airway smooth muscle.The Journal of general physiology · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To determine the relative contributions of Ca(2+) signaling and Ca(2+) sensitivity to the contractility of airway smooth muscle cells (SMCs), we compared the contractile responses of mouse and rat airways with the lung slice technique. Airway contraction was measured by monitoring changes in airway lumen area with phase-contrast microscopy, whereas changes in intracellular calcium concentration ([Ca(2+)](i)) of the SMCs were recorded with laser scanning microscopy. In mice and rats, methacholine (MCh) or serotonin induced concentration-dependent airway contraction and Ca(2+) oscillations in the SMCs. However, rat airways demonstrated greater contraction compared with mice, in response to agonist-induced Ca(2+) oscillations of a similar frequency. Because this indicates that rat airway SMCs have a higher Ca(2+) sensitivity compared with mice, we examined Ca(2+) sensitivity with Ca(2+)-permeabilized airway SMCs in which the [Ca(2+)](i) was experimentally controlled. In the absence of agonists, high [Ca(2+)](i) induced a sustained contraction in rat airways but only a transient contraction in mouse airways. This sustained contraction of rat airways was relaxed by Y-23672, a Rho kinase inhibitor, but not affected by GF-109203X, a PKC inhibitor. The subsequent exposure of Ca(2+)-permeabilized airway SMCs, with high [Ca(2+)](i), to MCh elicited a further contraction of rat airways and initiated a sustained contraction of mouse airways, without changing the [Ca(2+)](i) of the SMCs. Collectively, these results indicate that airway SMCs of rats have a substantially higher innate Ca(2+) sensitivity than mice and that this strongly influences the transduction of the frequency of Ca(2+) oscillations into the contractility of airway SMCs.
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