ArticleApplied and environmental microbiology2009
Identification of a Gene Cluster for the Biosynthesis of a Long, Galactose-Rich Exopolysaccharide in Lactobacillus rhamnosus GG and Functional Analysis of the Priming Glycosyltransferase.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 135 papers.
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135 citing papers in PubMed.
- Impact of orally administered lozenges with Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12 on the number of salivary mutans streptococci, amount of plaque, gingival inflammation and the oral microbiome in healthy adults.Clinical oral investigations · 2015Trial
- Integrative Phenotypic and Genomic Analysis Reveals Antimicrobial and Stress-Resistance Mechanisms ofMicroorganisms · 2026Article
- Exopolysaccharides of Lactobacillus crispatus mediate key balancing interactions with the vaginal mucosa.NPJ biofilms and microbiomes · 2026Article
- Lacticaseibacillus rhamnosus OF44 with Potent Antimicrobial Activity: Evidence from the Complete Genome and Phenotypic Analysis.Probiotics and antimicrobial proteins · 2026Article
- Genomic Assessment, Metabolic Profile Mapping, and Anti-Helicobacter pylori Activity of Lactococcus lactis SK2-659 from Thai Fermented Green Mustard (Pak-kad-dong).Probiotics and antimicrobial proteins · 2026Article
- Prophylactic and therapeutic effects of Limosilactobacillus reuteri E on 5-fluorouracil-induced intestinal mucositis in Caco-2 cells.Folia microbiologica · 2025Article
- Lactobacillus Bacteremia Following Dental Instrumentation in a Patient With a Bioprosthetic Valve.Cureus · 2025Article
- Probiogenomic analysis ofHeliyon · 2025Article
- Cell wall polysaccharide alters the surface electric potential ofCurrent research in microbial sciences · 2025Article
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- Coaggregation of oral pathogens by postbiotic lactobacilli.Journal of oral microbiology · 2025Article
- Molecular Mechanisms ofMicroorganisms · 2024Review
- Regulatory mechanisms and applications ofFrontiers in microbiology · 2024Review
- Functional genome analysis and anti-Helicobacter pylori activity of a novel bacteriocinogenic Lactococcus sp. NH2-7C from Thai fermented pork (Nham).Scientific reports · 2023Article
- Probiogenomic Analysis of Lactiplantibacillus sp. LM14-2 from Fermented Mussel (Hoi-dong), and Evaluation of its Cholesterol-lowering and Immunomodulation Effects.Probiotics and antimicrobial proteins · 2023Article
- New progress in the identifying regulatory factors of exopolysaccharide synthesis in lactic acid bacteria.World journal of microbiology & biotechnology · 2023Review
- Over-production of exopolysaccharide by Lacticaseibacillus rhamnosus CNCM I-3690 strain cutbacks its beneficial effect on the host.Scientific reports · 2023Article
- Exopolysaccharide is the potential effector ofFrontiers in microbiology · 2023Article
- Lactic Acid Bacteria (LAB): Autochthonous and Probiotic Microbes for Meat Preservation and Fortification.Foods (Basel, Switzerland) · 2022Review
- AI-2/LuxS Quorum Sensing System Promotes Biofilm Formation of Lactobacillus rhamnosus GG and Enhances the Resistance to Enterotoxigenic Escherichia coli in Germ-Free Zebrafish.Microbiology spectrum · 2022Article
75 more citing papers are in PubMed but not listed here.
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8 authors.
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Abstract
Cell surface polysaccharides have an established role as virulence factors in human bacterial pathogens. Less documented are the biosynthesis and biological functions of surface polysaccharides in beneficial bacteria. We identified a gene cluster that encodes the enzymes and regulatory and transporter proteins for the different steps in the biosynthesis of extracellular polysaccharides (EPS) of the well-documented probiotic strain Lactobacillus rhamnosus GG. Subsequent mutation of the welE gene, encoding the priming glycosyltransferase within this cluster, and comparative phenotypic analyses of wild-type versus mutant strains confirmed the specific function of this gene cluster in the biosynthesis of high-molecular-weight, galactose-rich heteropolymeric EPS molecules. The phenotypic analyses included monomer composition determination, estimation of the polymer length of the isolated EPS molecules, and single-molecule force spectroscopy of the surface polysaccharides. Further characterization of the welE mutant also showed that deprivation of these long, galactose-rich EPS molecules results in an increased adherence and biofilm formation capacity of L. rhamnosus GG, possibly because of less shielding of adhesins such as fimbria-like structures.
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